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Formulation and evaluation of a novel megalomeric microemulsion from tamarind seed xyloglucan-megalosaccharides for improved high-dose quercetin delivery
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| Title: | Formulation and evaluation of a novel megalomeric microemulsion from tamarind seed xyloglucan-megalosaccharides for improved high-dose quercetin delivery |
| Authors: | Lang, Weeranuch Browse this author | | Mondol, Debashish Browse this author | | Trakooncharoenvit, Aphichat Browse this author | | Tagami, Takayoshi Browse this author →KAKEN DB | | Okuyama, Masayuki Browse this author →KAKEN DB | | Hira, Tohru Browse this author →KAKEN DB | | Sakairi, Nobuo Browse this author →KAKEN DB | | Kimura, Atsuo Browse this author →KAKEN DB |
| Keywords: | Tamarind seed xyloglucan | | Megalosaccharide | | Antioxidative flavonoid | | Cosurfactant | | Microemulsion formulation | | Self-emulsifying drug delivery system |
| Issue Date: | Apr-2023 |
| Publisher: | Elsevier |
| Journal Title: | Food Hydrocolloids |
| Volume: | 137 |
| Start Page: | 108430 |
| Publisher DOI: | 10.1016/j.foodhyd.2022.108430 |
| Abstract: | Megalomeric microemulsion is a new term referring to lipid-based formulation using amphiphilic megalosaccharide as a coexcipient. Quercetin is a dose-dependent bioactive compound and has promising therapeutic potential, but its low water solubility and permeability restrict its treatment efficacy. We aimed to formulate high-dose quercetin loaded into colloidal micelles by the self-micro emulsifying system (SMES) in combination with Tween 80, isopropyl myristate, and xyloglucan megalosaccharide (X-MS). X-MS is a moderate-size heterologous saccharide obtained from enzymatic cleavage of tamarind seed xyloglucan. X-MSs with an average degree of polymerization of 16 and 56 were investigated to bearing their surface hydrophobic interaction with a fluorescence probe 6-(p-toluidino)-2-naphthalene-6-sulfonate yielded the binding constant values of 127 and 180 M-1, respectively and X-MS itself displayed a slight effect on quercetin binding. However, the implementation of X-MSs toward SMES was highly compatible because X-MS molecules were confined in micellular solutions. Consequently, X-MSs improved the quercetin loading from 1 to 2 to 12.5-17.7 mg/mL based on the composition ratio, X-MS chain lengths, and X-MS concentrations (0.15-3.0%, w/v) and stabilized the quercetin-loaded oil-in-water SMES. The optical appearances were transparent yellow containing uniformly fine droplets with diameters of 11-12 nm. In vitro radical scavenging activity tests with 2,2-diphenyl-1-picrylhydrazyl showed that the megalomeric microemulsions improved the half-maximal inhibitory concentration (IC50 = 22-24 μg/mL) over that of the X-MS-free microemulsion. This study provided a new approach of liquid supplementation from commercially unavailable-size xyloglucan to be a promising added-value agent for oral uptake of quercetin. |
| Rights: | © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/91441 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: Lang Weeranuch
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