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Nonreducing terminal chimeric isomaltomegalosaccharide and its integration with azoreductase for the remediation of soil-contaminated lipophilic azo dyes
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| Title: | Nonreducing terminal chimeric isomaltomegalosaccharide and its integration with azoreductase for the remediation of soil-contaminated lipophilic azo dyes |
| Authors: | Lang, Weeranuch Browse this author | | Sirisansaneeyakul, Sarote Browse this author | | Tagami, Takayoshi Browse this author →KAKEN DB | | Kang, Hye-Jin Browse this author | | Okuyama, Masayuki Browse this author →KAKEN DB | | Sakairi, Nobuo Browse this author →KAKEN DB | | Kimura, Atsuo Browse this author →KAKEN DB |
| Keywords: | Azobenzene dye | | Biological treatment | | Chimeric structure | | Dye-contaminated soil remediation | | Saccharide solubilizer | | Methyl yellow |
| Issue Date: | 1-Apr-2023 |
| Publisher: | Elsevier |
| Journal Title: | Carbohydrate Polymers |
| Volume: | 305 |
| Start Page: | 120565 |
| Publisher DOI: | 10.1016/j.carbpol.2023.120565 |
| Abstract: | Lipophilic azo dyes are practically water-insoluble, and their dissolution by organic solvents and surfactants is harmful to biological treatment with living cells and enzymes. This study aimed to evaluate the feasibility of a newly synthesized nonreducing terminal chimeric isomaltomegalosaccharide (N-IMS) as a nontoxic solubilizer of four simulated lipophilic azo dye wastes for enzymatic degradation. N-IMS bearing a helical α-(1 → 4)-glucosidic segment derived from a donor substrate α-cyclodextrin was produced by a coupling reaction of cyclodextrin glucanotransferase. Inclusion complexing by N-IMS overcame the solubility issue with equilibrium constants of 1786-242 M-1 (methyl yellow > ethyl red > methyl red > azo violet). Circular dichroism spectra revealed the axial alignment of the aromatic rings in the N-IMS cavity, while UV-visible absorption quenching revealed that the azo bond of methyl yellow was particularly induced. Desorption of the dyes from acidic and neutral soils was specific to aqueous organic over alkali extraction. The dissolution kinetics of the incorporated dyes followed a sigmoid pattern facilitating the subsequent decolorization process with azoreductase. It was demonstrated that after soil extraction, the solid dyes dissolved with N-IMS assistance and spontaneously digested by coupled azoreductase/glucose dehydrogenase (for a cofactor regeneration system) with the liberation of the corresponding aromatic amine. |
| Rights: | © 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/91442 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: Lang Weeranuch
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