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Hokkaido University Collection of Scholarly and Academic Papers >
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Identification and characterization of extracellular GH3 beta-glucosidase from the pink snow mold fungus, Microdochium nivale
| Title: | Identification and characterization of extracellular GH3 beta-glucosidase from the pink snow mold fungus, Microdochium nivale |
| Authors: | Ota, Tomoya Browse this author | | Saburi, Wataru Browse this author →KAKEN DB | | Jewell, Linda Elizabeth Browse this author | | Hsiang, Tom Browse this author | | Imai, Ryozo Browse this author | | Mori, Haruhide Browse this author →KAKEN DB |
| Keywords: | Microdochium nivale | | beta-glucosidase | | glycoside hydrolase family 3 | | substrate specificity | | transglucosylation |
| Issue Date: | 13-Apr-2023 |
| Publisher: | Oxford University Press |
| Journal Title: | Bioscience biotechnology and biochemistry |
| Volume: | 87 |
| Issue: | 7 |
| Start Page: | 707 |
| End Page: | 716 |
| Publisher DOI: | 10.1093/bbb/zbad044 |
| Abstract: | Glycoside hydrolase family 3 (GH3) beta-glucosidase exists in many filamentous fungi. In phytopathogenic fungi, it is involved in fungal growth and pathogenicity. Microdochium nivale is a severe phytopathogenic fungus of grasses and cereals and is the causal agent of pink snow mold, but its beta-glucosidase has not been identified. In this study, a GH3 beta-glucosidase of M. nivale (MnBG3A) was identified and characterized. Among various p-nitrophenyl beta-glycosides, MnBG3A showed activity on d-glucoside (pNP-Glc) and slight activity on d-xyloside. In the pNP-Glc hydrolysis, substrate inhibition occurred (K-is = 1.6 m m), and d-glucose caused competitive inhibition (K-i = 0.5 m m). MnBG3A acted on beta-glucobioses with beta 1-3, -6, -4, and -2 linkages, in descending order of k(cat)/K-m. In contrast, the regioselectivity for newly formed products was limited to beta 1-6 linkage. MnBG3A has similar features to those of beta-glucosidases from Aspergillus spp., but higher sensitivity to inhibitory effects. |
| Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in Bioscience biotechnology and biochemistry following peer review. The version of record Tomoya Ota and others, Identification and characterization of extracellular GH3 β-glucosidase from the pink snow mold fungus, Microdochium nivale, Bioscience, Biotechnology, and Biochemistry, Volume 87, Issue 7, July 2023, Pages 707–716, is available online at: https://doi.org/10.1093/bbb/zbad044. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/91655 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 太田 智也
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