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Morphological Study for the Osteocytes in Podoplanin-Conditional Knockout Mice
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| Title: | Morphological Study for the Osteocytes in Podoplanin-Conditional Knockout Mice |
| Other Titles: | ポドプラニンコンディショナルノックアウトマウスにおける骨細胞の形態学的研究 |
| Authors: | 大澤, 杏子 Browse this author |
| Keywords: | Podoplanin | | cKO | | Osteocyte |
| Issue Date: | 25-Mar-2024 |
| Publisher: | Hokkaido University |
| Abstract: | We generated podoplanin-conditional knockout mice where the floxed podoplanin exon3 was deleted by the Dmp-1-driven Cre (Dmp1-Cre;PdpnΔ/Δ) and investigated the cell process elongation of podoplanin-deficient mouse osteocyte in vitro and in vivo. The expression of podoplanin is found in odontoblasts while not observed in odontoblasts of Dmp1-Cre;PdpnΔ/Δ mice, indicating that the conditional knockout of podoplanin in Dmp1-expressing cells in Dmp1-Cre;PdpnΔ/Δ mice is successful. There were no differences in the growth of wild-type and Dmp1-Cre;PdpnΔ/Δ mice, and no differences in calci-fication and alkaline phosphatase activity in cultured calvarial osteoblasts of the wild-type and Dmp1-Cre;PdpnΔ/Δ mice,in total this suggests that the podoplanin-cKO has no effect on generation of the bone. The cell process elongation was sup-pressed in cultured calvarial osteoblasts of Dmp1-Cre;PdpnΔ/Δ mice compared with wild-type mice. In the electron micro-scopic study, there were no morphological differences in bone matrix formation and osteocyte distribution in Dmp1-Cre;PdpnΔ/Δ and wild-type mice, whereas the cell process formation was sparser and the network with neighboring cells was more deficient in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. In the quantitative analysis, the number and thickness of the cell processes were significantly smaller and thinner in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. This could sug-gest that podoplanin plays a role in the formation of the osteocyte network created by the cell process elongation. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第15933号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 教授 佐藤 嘉晃, 教授 網塚 憲生, 教授 飯村 忠浩 |
| Degree Affiliation: | 歯学院(口腔医学専攻) |
| (Relation)isversionof: | Journal of Hard Tissue Biology, 2023, 32 巻, 4 号, p. 213-222 | | https://doi.org/10.2485/jhtb.32.213 |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/92174 |
| Appears in Collections: | 課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
学位論文 (Theses) > 博士 （歯学）
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