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Rapid and Reliable Steatosis Rat Model Shrsp5-Dmcr for Cold Storage Experiment

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Title: Rapid and Reliable Steatosis Rat Model Shrsp5-Dmcr for Cold Storage Experiment
Authors: Fukai, Moto Browse this author →KAKEN DB
Sugimori, Hiroyuki Browse this author
Sakamoto, Sodai Browse this author
Shibata, Kengo Browse this author
Kameda, Hiroyuki Browse this author
Ishikawa, Takahisa Browse this author
Kawamura, Norio Browse this author
Fujiyoshi, Masato Browse this author
Fujiyoshi, Sunao Browse this author
Kudo, Kohsuke Browse this author
Shimamura, Tsuyoshi Browse this author
Taketomi, Akinobu Browse this author →KAKEN DB
Issue Date: May-2023
Publisher: Elsevier
Journal Title: Transplantation proceedings
Volume: 55
Issue: 4
Start Page: 1032
End Page: 1035
Publisher DOI: 10.1016/j.transproceed.2023.02.063
Abstract: Interventions for liver grafts with moderate macrovesicular steatosis have been important in enlarging donor pools. Here, we tested a high-fat and cholesterol (HFC) diet to create a steatosis model for cold hepatic preservation and reperfusion experiments. The aim of the present study was to assess the steatosis model's reliability and to show the resulting graft's quality for cold preservation and reperfusion experiment. Male SHRSP5-Dmcr rats were raised with an HFC diet for up to 2 weeks. The fat content was evaluated using magnetic resonance imaging (MRI) proton density fat fraction (PDFF). The nonalcoholic fatty liver disease activity score (NAS) was evaluated after excision. Steatosis created by 2 weeks of HFC diet was subjected to 24-hour cold storage in the University of Wisconsin and the original test solution (new sol.). Grafts were applied to isolated perfused rat livers for simulating reperfusion. The NAS were 2.2 (HFC 5 days), 3.3 (HFC 1 week), and 5.0 (HFC 2 weeks). Ballooning and fibrosis were not observed in any group. An MRI-PDFF showed 0.2 (HFC 0 days), 12.0 (HFC 1 week), and 18.9 (HFC 2 weeks). The NAS and MRI-PDFF values correlated. Many indices in the isolated perfused rat liver experiment tended to improve in the new sol. group but were insufficient. Although the new sol. failed to be effective, it acted at multiple sites under difficult conditions. In conclusion, the HFC diet for 2 weeks in SHRSP5-Dmcr rats, together with MRI-PDFF evaluation, is a reliable method for creating simple steatosis and provides good-quality cold preservation and reperfusion experiments.
Rights: © 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 武冨 紹信

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