| Title: | Rapid and Reliable Steatosis Rat Model Shrsp5-Dmcr for Cold Storage Experiment |
| Authors: | Fukai, Moto Browse this author →KAKEN DB |
| Sugimori, Hiroyuki Browse this author |
| Sakamoto, Sodai Browse this author |
| Shibata, Kengo Browse this author |
| Kameda, Hiroyuki Browse this author |
| Ishikawa, Takahisa Browse this author |
| Kawamura, Norio Browse this author |
| Fujiyoshi, Masato Browse this author |
| Fujiyoshi, Sunao Browse this author |
| Kudo, Kohsuke Browse this author |
| Shimamura, Tsuyoshi Browse this author |
| Taketomi, Akinobu Browse this author →KAKEN DB |
| Issue Date: | May-2023 |
| Publisher: | Elsevier |
| Journal Title: | Transplantation proceedings |
| Volume: | 55 |
| Issue: | 4 |
| Start Page: | 1032 |
| End Page: | 1035 |
| Publisher DOI: | 10.1016/j.transproceed.2023.02.063 |
| Abstract: | Interventions for liver grafts with moderate macrovesicular steatosis have been important in enlarging donor pools. Here, we tested a high-fat and cholesterol (HFC) diet to create a steatosis model for cold hepatic preservation and reperfusion experiments. The aim of the present study was to assess the steatosis model's reliability and to show the resulting graft's quality for cold preservation and reperfusion experiment. Male SHRSP5-Dmcr rats were raised with an HFC diet for up to 2 weeks. The fat content was evaluated using magnetic resonance imaging (MRI) proton density fat fraction (PDFF). The nonalcoholic fatty liver disease activity score (NAS) was evaluated after excision. Steatosis created by 2 weeks of HFC diet was subjected to 24-hour cold storage in the University of Wisconsin and the original test solution (new sol.). Grafts were applied to isolated perfused rat livers for simulating reperfusion. The NAS were 2.2 (HFC 5 days), 3.3 (HFC 1 week), and 5.0 (HFC 2 weeks). Ballooning and fibrosis were not observed in any group. An MRI-PDFF showed 0.2 (HFC 0 days), 12.0 (HFC 1 week), and 18.9 (HFC 2 weeks). The NAS and MRI-PDFF values correlated. Many indices in the isolated perfused rat liver experiment tended to improve in the new sol. group but were insufficient. Although the new sol. failed to be effective, it acted at multiple sites under difficult conditions. In conclusion, the HFC diet for 2 weeks in SHRSP5-Dmcr rats, together with MRI-PDFF evaluation, is a reliable method for creating simple steatosis and provides good-quality cold preservation and reperfusion experiments. |
| Rights: | © 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/92185 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
