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Hokkaido University Collection of Scholarly and Academic Papers >
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Periodontal tissue regeneration by recombinant human collagen peptide granules applied with beta-tricalcium phosphate fine particles
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| Title: | Periodontal tissue regeneration by recombinant human collagen peptide granules applied with beta-tricalcium phosphate fine particles |
| Authors: | Yoshino, Yuto Browse this author | | Miyaji, Hirofumi Browse this author →KAKEN DB | | Nishida, Erika Browse this author →KAKEN DB | | Kanemoto, Yukimi Browse this author →KAKEN DB | | Hamamoto, Asako Browse this author | | Kato, Akihito Browse this author →KAKEN DB | | Sugaya, Tsutomu Browse this author →KAKEN DB | | Akasaka, Tsukasa Browse this author →KAKEN DB |
| Keywords: | Animals | | Biocompatible materials | | Bone formation | | Immunohistochemistry | | Periodontal ligament |
| Issue Date: | Mar-2023 |
| Publisher: | Elsevier |
| Journal Title: | Journal of oral biosciences |
| Volume: | 65 |
| Issue: | 1 |
| Start Page: | 62 |
| End Page: | 71 |
| Publisher DOI: | 10.1016/j.job.2023.01.002 |
| PMID: | 36669699 |
| Abstract: | Objectives: Recombinant human collagen peptide (RCP) is a recombinantly created xeno-free biomaterial enriched in arginine-glycine-aspartic acid sequences with good processability whose use for regenera-tive medicine applications is under investigation. The biocompatibility and osteogenic ability of RCP granules combined with f3-tricalcium phosphate (TCP) submicron particles (f3-TCP/RCP) were recently demonstrated. In the present study, f3-TCP/RCP was implanted into experimental periodontal tissue defects created in beagles to investigate its regenerative effects. Methods: An RCP solution was lyophilized, granulated, and thermally cross-linked into particles approximately 1 mm in diameter. f3-TCP dispersion (1 wt%; 500 mu L) was added to 100 mg of RCP granules to form f3-TCP/RCP. A three-walled intrabony defect (5 mm x 3 mm x 4 mm) was created on the mesial side of the mandibular first molar and filled with f3-TCP/RCP.Results: A micro-computed tomography image analysis performed at 8 weeks postoperative showed a significantly greater amount of new bone after f3-TCP/RCP grafting (2.2-fold, P < 0.05) than after no grafting. Histological findings showed that the transplanted f3-TCP/RCP induced active bone-like tissue formation including tartaric acid-resistant acid phosphatase-and OCN-positive cells as well as bio-absorbability. Ankylosis did not occur, and periostin-positive periodontal ligament-like tissue formation was observed. Histological measurements performed at 8 weeks postoperative revealed that f3-TCP/RCP implantation formed 1.7-fold more bone-like tissue and 2.1-fold more periodontal ligament-like tissue than the control condition and significantly suppressed gingival recession and epithelial downgrowth (P < 0.05).Conclusions: f3-TCP/RCP implantation promoted bone-like and periodontal ligament-like tissue forma-tion, suggesting its efficacy as a periodontal tissue regenerative material. |
| Rights: | ©2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/92679 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮治 裕史
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