|
Hokkaido University Collection of Scholarly and Academic Papers >
Theses >
博士 （保健科学） >
Studies on the changes of oxidative stress and autophagy in the hearts of streptozotocin-induced early diabetes rats, and the improvement effects by azuki bean (Vigna angularis) extract
|
Files in This Item:
|
|
|
Related Items in HUSCAP:
|
| Title: | Studies on the changes of oxidative stress and autophagy in the hearts of streptozotocin-induced early diabetes rats, and the improvement effects by azuki bean (Vigna angularis) extract |
| Other Titles: | ストレプトゾトシン誘導初期糖尿病ラットの心臓における酸化ストレスおよびオートファジーの変化と小豆抽出物による改善効果に関する研究 |
| Authors: | 冨原, 朋美1 Browse this author |
| Authors(alt): | 北, 朋美1 |
| Keywords: | polyphenols | | diabetes | | heart | | oxidative damage | | autophagy |
| Issue Date: | 28-Jun-2019 |
| Publisher: | Hokkaido University |
| Abstract: | I examined the changes in oxidative stress and autophagy function of the heart of rats at the early stages of streptozotocin (STZ)-induced diabetes, and whether azuki bean extract (ABE) could influence these changes. The experimental diabetic rats received 0 or 40 mg/kg of ABE orally for 4 weeks, whereas the control group rats received distilled water. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) and expression of proteins associated with peroxisomal FA β-oxidation, oxidative stress markers as well as autophagy-related proteins were examined. The levels of peroxisomal ACOX1 and catalase of the diabetic groups were significantly higher than those in the control group. ABE did not effect on the increased level of these enzymes. The levels of p62, phosphorylated-p62 (p-p62) and HO-1 in the STZ group were significantly higher than those in the control group, and the levels of p-p62, HO-1, and 8-OHdG were significantly lower by ABE administration as compared with those in the STZ group. The increase in peroxisomal ACOX1 and catalase in the heart of STZ-induced early-diabetic rats suggested that peroxisomal FA-oxidation is enhanced in the early-diabetic heart. Furthermore, the increase in the levels of oxidative stress markers p62, p-p62 and HO-1 suggested that the heart of early-stage diabetes is under strong oxidative stress. ABE has antioxidant effects and protects from oxidative damage of the heart of STZ-induced early-diabetic rats. In addition to stress markers, p62 and p-p62 are known to be responsible for transporting substances such as damaged organelles and proteins that will be degraded by autophagy, to autophagosome. Marked accumulation of p62 and p-p62 was observed in STZ-induced early-diabetic rats. Thus, I examined next the changes in autophagy function of the heart of rats at the early stages of STZ-induced diabetes. The levels of mTOR and Beclin1 proteins that are rerated with the autophagy induction did not change in any of the groups. The levels of O-GlcNAc modification and LAMP2 protein related to the fusion of autophagosome and lysosome were significantly increased in the STZ group compared to the control group. ABE has the effect of suppressing the increase in the levels of LAMP2 and O-GlcNAc modification. The present study showed that the STZ-induced early diabetes increases the levels of proteins related with peroxisomal FA β-oxidation, oxidative stress markers, and the fusion of autophagosomes and lysosomes in hearts. ABE protects diabetic hearts from oxidative damage and has the effect of maintaining autophagy function from the early stage of diabetes. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第13705号 |
| Degree Level: | 博士 |
| Degree Discipline: | 保健科学 |
| Examination Committee Members: | (主査) 教授 惠 淑萍, 准教授 蔵崎 正明（地球環境科学研究院）, 教授 齋藤 健 |
| Degree Affiliation: | 保健科学院（保健科学専攻） |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/92689 |
| Appears in Collections: | 学位論文 (Theses) > 博士 （保健科学）
課程博士 (Doctorate by way of Advanced Course) > 保健科学院(Graduate School of Health Sciences)
|
|
