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Hokkaido University Collection of Scholarly and Academic Papers >
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Hydrolysis-transglycosylation of sucrose and production of β-(2→1)-fructan by inulosucrase from Neobacillus drentensis 57N
| Title: | Hydrolysis-transglycosylation of sucrose and production of β-(2→1)-fructan by inulosucrase from Neobacillus drentensis 57N |
| Authors: | Kido, Yusuke Browse this author | | Saburi, Wataru Browse this author →KAKEN DB | | Nagura, Taizo Browse this author | | Mori, Haruhide Browse this author →KAKEN DB |
| Keywords: | inulosucrase | | inulin | | sucrose | | Neobacillus drentensis 57N | | glycoside hydrolase family 68 |
| Issue Date: | 25-Jul-2023 |
| Publisher: | Oxford University Press |
| Journal Title: | Bioscience biotechnology and biochemistry |
| Volume: | 87 |
| Issue: | 10 |
| Start Page: | 1169 |
| End Page: | 1182 |
| Publisher DOI: | 10.1093/bbb/zbad100 |
| Abstract: | Inulin, beta-(2 -> 1)-fructan, is a beneficial polysaccharide used as a functional food ingredient. Microbial inulosucrases (ISs), catalyzing beta-(2 -> 1)-transfructosylation, produce beta-(2 -> 1)-fructan from sucrose. In this study, we identified a new IS (NdIS) from the soil isolate, Neobacillus drentensis 57N. Sequence analysis revealed that, like other Bacillaceae ISs, NdIS consists of a glycoside hydrolase family 68 domain and shares most of the 1-kestose-binding residues of the archaeal IS, InuHj. Native and recombinant NdIS were characterized. NdIS is a homotetramer. It does not require calcium for activity. High performance liquid chromatography and C-13-nuclear magnetic resonance indicated that NdIS catalyzed the hydrolysis and beta-(2 -> 1)-transfructosylation of sucrose to synthesize beta-(2 -> 1)-fructan with chain lengths of 42 or more residues. The rate dependence on sucrose concentration followed hydrolysis-transglycosylation kinetics, and a 50% transglycosylation ratio was obtained at 344 mm sucrose. These results suggest that transfructosylation from sucrose to beta-(2 -> 1)-fructan occurs predominantly to elongate the fructan chain because sucrose is an unfavorable acceptor. [GRAPHICS] . |
| Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in Bioscience biotechnology and biochemistry following peer review. The version of record Yusuke Kido, Wataru Saburi, Taizo Nagura, Haruhide Mori, Hydrolysis-transglycosylation of sucrose and production of β-(2→1)-fructan by inulosucrase from Neobacillus drentensis 57N, Bioscience, Biotechnology, and Biochemistry, Volume 87, Issue 10, October 2023, Pages 1169–1182, is available online at: https://doi.org/10.1093/bbb/zbad100. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/92827 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 森 春英
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