HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >

Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography

Files in This Item:
JCA05-1916R4-REVISED.pdf433.29 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/10521

Title: Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography
Authors: Takegawa, Yasuhiro Browse this author →KAKEN DB
Deguchi, Kisaburo2 Browse this author
Keira, Takuro Browse this author
Ito, Hiroki Browse this author
Nakagawa, Hiroaki Browse this author
Nishimura, Shin-Ichiro Browse this author →KAKEN DB
Authors(alt): 出口, 喜三郎2
Keywords: zwitterion-interaction column
hydrophilic-interaction chromatography
oligosaccharides
glycopeptides
mass spectrometry
Issue Date: 28-Apr-2006
Publisher: Elsevier
Journal Title: Journal of Chromatography A
Volume: 1113
Issue: 1-2
Start Page: 177
End Page: 181
Publisher DOI: 10.1016/j.chroma.2006.02.010
PMID: 16503336
Abstract: Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns. A zwitterionic type of hydrophilic-interaction chromatography column with sulfobetaine groups (called ZIC-HILIC column) was first applied to the separation of 2-aminopyridine derivatized (PA) N-glycans and tryptic peptides of human serum immunoglobulin G (IgG). It is shown that the ZIC-HILIC column has high capability for structural recognition of isomeric N-glycans as well as high selectivity for glycopeptides. The former feature (i.e., structural recognition) was proven by sufficient separation of neutral PA N-glycan isomers, which are usually difficult to separate on NP and RP columns. In addition, it is noteworthy that IgG glycopeptides consisting of isomeric N-glycans and the same peptide sequences can be sufficiently separated on a ZIC-HILIC column. The latter feature (i.e., selectivity) was also demonstrated by easily separating two peptide groups with/without N-glycans. Thus, we note that the ZIC-HILIC column is highly promising for a simple analysis of N-glycans and N-glycopeptide samples. (c) 2006 Elsevier B.V. All rights reserved.
Relation: http://www.sciencedirect.com/science/journal/00219673
Type: article (author version)
URI: http://hdl.handle.net/2115/10521
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 出口 喜三郎

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University