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Importance of specific hydrogen bonds of archaeal rhodopsins for the binding to the transducer protein†

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タイトル: Importance of specific hydrogen bonds of archaeal rhodopsins for the binding to the transducer protein†
著者: Sudo, Yuki 著作を一覧する
Yamabi, Masaki 著作を一覧する
Kato, Shinnosuke 著作を一覧する
Hasegawa, Chisa 著作を一覧する
Iwamoto, Masayuki 著作を一覧する
Shimono, Kazumi 著作を一覧する
Kamo, Naoki 著作を一覧する
キーワード: photo-sensor
protein-protein interaction,
ion pump
sensory rhodopsin
発行日: 2006年 4月 7日
巻: 357
号: 4
開始ページ: 1274
終了ページ: 1282
出版社 DOI: 10.1016/j.jmb.2006.01.061
抄録: Four rhodopsins, bacteriorhodopsin (bR), halorhodopsin (hR), sensory rhodopsin (sR) and phoborhodopsin (pR) exist in archaeal membranes. bR and hR work as a light-driven ion pump. sR and pR work as a photo-sensor of phototaxis, and form signaling complexes in membranes with their respective cognate transducer proteins HtrI (with sR) and HtrII (with pR), through which light signals are transmitted to cytoplasm. What is the determining factor(s) of the specific binding to form the complex? Binding of the wild or mutated rhodopsins with HtrII was measured by Isothermal titration calorimetric analysis (ITC). bR and hR could not bind with HtrII. On the other hand, sR could bind to HtrII, although the dissociation constant (KD) was about 100-times larger than that of pR. X-ray crystallographic structure of the pR/HtrII complex (Gordeliy et al. Nature 419, 484-487) revealed the formation of two specific hydrogen bonds whose pairs are Tyr199pR/Asn74HtrII and Thr189pR/Glu43HtrII/Ser62HtrII. To investigate the importance of these hydrogen bonds, the KD for the binding of various mutants of bR, hR, sR and pR with HtrII was estimated by ITC. The KD value of T189VpR/Y199FpR, double mutant/HtrII complex, was about 100-fold larger than that of the wild-type pR whose KD value was 0.16 μM. On the other hand, bR and hR double mutants, P200TbR/V210YbR and P240ThR/F250YhR, were able to bind with HtrII. The KD value of these complexes was estimated to be 60.1 ± 10.7 μM for bR and to be 29.1 ± 6.1 μM for hR while the wild bR and hR did not bind with HtrII. We concluded that these two specific hydrogen bonds played important roles in the binding between the rhodopsins and transducer protein.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 加茂 直樹


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