HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
獣医学研究科  >
雑誌発表論文等  >

Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells

JRR47_19.pdf331.49 kBPDF見る/開く

タイトル: Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells
著者: TAKAHASHI, Eriko 著作を一覧する
INANAMI, Osamu 著作を一覧する
ASANUMA, Taketoshi 著作を一覧する
KUWABARA, Mikinori 著作を一覧する
キーワード: Ceramide
Fumonisin B1
X irradiation
発行日: 2006年 3月30日
出版者: 日本放射線影響学会
誌名: Journal of Radiation Research
巻: 47
号: 1
開始ページ: 19
終了ページ: 25
出版社 DOI: 10.1269/jrr.47.19
抄録: In the present study, using inhibitors of ceramide synthase (fumonisin B1), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B1 and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 桑原幹典


本サイトに関するご意見・お問い合わせは repo at へお願いします。 - 北海道大学