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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells
| Title: | Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells |
| Authors: | TAKAHASHI, Eriko Browse this author | | INANAMI, Osamu Browse this author →KAKEN DB | | ASANUMA, Taketoshi Browse this author | | KUWABARA, Mikinori Browse this author |
| Keywords: | Ceramide | | Fumonisin B1 | | MOLT-4 | | Sphingomyelinase | | X irradiation |
| Issue Date: | 30-Mar-2006 |
| Publisher: | 日本放射線影響学会 |
| Journal Title: | Journal of Radiation Research |
| Volume: | 47 |
| Issue: | 1 |
| Start Page: | 19 |
| End Page: | 25 |
| Publisher DOI: | 10.1269/jrr.47.19 |
| Abstract: | In the present study, using inhibitors of ceramide synthase (fumonisin B1), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B1 and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/11387 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 桑原幹典
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