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Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/11387

Title: Effects of Ceramide Inhibition on Radiation-induced Apoptosis in Human Leukemia MOLT-4 Cells
Authors: TAKAHASHI, Eriko Browse this author
INANAMI, Osamu Browse this author →KAKEN DB
ASANUMA, Taketoshi Browse this author
KUWABARA, Mikinori Browse this author
Keywords: Ceramide
Fumonisin B1
MOLT-4
Sphingomyelinase
X irradiation
Issue Date: 30-Mar-2006
Publisher: 日本放射線影響学会
Journal Title: Journal of Radiation Research
Volume: 47
Issue: 1
Start Page: 19
End Page: 25
Publisher DOI: 10.1269/jrr.47.19
Abstract: In the present study, using inhibitors of ceramide synthase (fumonisin B1), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B1 and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells.
Type: article
URI: http://hdl.handle.net/2115/11387
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 桑原幹典

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