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A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine.
| Title: | A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine. |
| Authors: | Iio, Kaori Browse this author | | Nakauchi, Mina Browse this author | | Yamagami, Sayaka Browse this author | | Tsutsumi, Makiko Browse this author | | Hori, Hiroshi Browse this author | | Naruse, Kiyoshi Browse this author | | Mitani, Hiroshi Browse this author | | Shima, Akihiro Browse this author | | Suzuki, Norio Browse this author |
| Keywords: | guanylyl cyclase | | medaka fish | | intestine | | enterotoxin | | transcription | | GC-C |
| Issue Date: | Apr-2005 |
| Publisher: | Elsevier |
| Journal Title: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
| Volume: | 140 |
| Issue: | 4 |
| Start Page: | 569 |
| End Page: | 578 |
| Publisher DOI: | 10.1016/j.cbpc.2004.12.006 |
| PMID: | 15763512 |
| Abstract: | A novel membrane guanylyl cyclase (GC), OlGC9, was identified in the intestine of the medaka fish Oryzias latipes by the isolation of a full-length cDNA clone (3783 bp). Phylogenetic analysis indicated that OlGC9 belongs in the enterotoxin/guanylin receptor membrane GC subfamily. The nucleotide and deduced amino acid sequences of OlGC9 were highly homologous to those of OlGC6, another enterotoxin/guanylin receptor membrane GC in medaka fish. Linkage analysis of the medaka fish chromosome demonstrated that the OlGC9 gene was mapped to LG8, which distinguishes it from the OlGC6 gene. Determination of the cGMP concentrations in COS-7 cells expressed with OlGC9 indicated that Escherichia coli heat-stable enterotoxin (STa) stimulated the activity of OlGC9 in a concentration-dependent manner, although it did not activate the OlGC6 expressed in the COS-7 cells. The 5â²-flanking region of the OlGC9 gene important for its transcription was partially determined using both CACO-2 cells and COS-1 cells, and was not found to be conserved with respect to either the mammalian GC-C gene or the OlGC6 gene. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/13465 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 範男
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