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A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine.

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Title: A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine.
Authors: Iio, Kaori Browse this author
Nakauchi, Mina Browse this author
Yamagami, Sayaka Browse this author
Tsutsumi, Makiko Browse this author
Hori, Hiroshi Browse this author
Naruse, Kiyoshi Browse this author
Mitani, Hiroshi Browse this author
Shima, Akihiro Browse this author
Suzuki, Norio Browse this author
Keywords: guanylyl cyclase
medaka fish
intestine
enterotoxin
transcription
GC-C
Issue Date: Apr-2005
Publisher: Elsevier
Journal Title: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Volume: 140
Issue: 4
Start Page: 569
End Page: 578
Publisher DOI: 10.1016/j.cbpc.2004.12.006
PMID: 15763512
Abstract: A novel membrane guanylyl cyclase (GC), OlGC9, was identified in the intestine of the medaka fish Oryzias latipes by the isolation of a full-length cDNA clone (3783 bp). Phylogenetic analysis indicated that OlGC9 belongs in the enterotoxin/guanylin receptor membrane GC subfamily. The nucleotide and deduced amino acid sequences of OlGC9 were highly homologous to those of OlGC6, another enterotoxin/guanylin receptor membrane GC in medaka fish. Linkage analysis of the medaka fish chromosome demonstrated that the OlGC9 gene was mapped to LG8, which distinguishes it from the OlGC6 gene. Determination of the cGMP concentrations in COS-7 cells expressed with OlGC9 indicated that Escherichia coli heat-stable enterotoxin (STa) stimulated the activity of OlGC9 in a concentration-dependent manner, although it did not activate the OlGC6 expressed in the COS-7 cells. The 5′-flanking region of the OlGC9 gene important for its transcription was partially determined using both CACO-2 cells and COS-1 cells, and was not found to be conserved with respect to either the mammalian GC-C gene or the OlGC6 gene.
Type: article (author version)
URI: http://hdl.handle.net/2115/13465
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 範男

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