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Involvement of general transcriptional coactivator PC4 in the transcription of medaka fish intestine-specific membrane guanylyl cyclase gene (OlGC6).

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Title: Involvement of general transcriptional coactivator PC4 in the transcription of medaka fish intestine-specific membrane guanylyl cyclase gene (OlGC6).
Authors: Nakauchi, Mina Browse this author
Yoshino, Ken-ichi Browse this author
Yonezawa, Kazuyoshi Browse this author
Suzuki, Norio Browse this author
Keywords: coactivator
intestine-specific membrane guanylyl cyclase
Medaka fish
positive cofactor 4, transcription
Issue Date: Apr-2005
Publisher: Oxford University Press
Journal Title: Journal of Biochemistry
Volume: 137
Issue: 4
Start Page: 509
End Page: 515
Publisher DOI: 10.1093/jb/mvi066
PMID: 15858175
Abstract: A recent study showed that the AGACCTTTGC nucleotides sequence (between –90 and –81) contained in the cis-regulatory element in an intestine-specific membrane guanylyl cyclase gene, OlGC6, of the medaka fish, Oryzias latipes, are important for the transcription of the gene in mammalian cultured cell line and in medaka fish. Using sequence-specific DNA affinity chromatography, we purified a cis-regulatory element–binding protein from a medaka fish intestinal nuclear extract and used mass spectrometry to identify it as a medaka fish homologue of general transcriptional coactivator PC4, which we designated as OlPC4. The expression of the OlPC4 gene was detected in embryos, as well as in a large variety of tissues of adult medaka fish. Using a 17-kDa recombinant OlPC4, we carried out an ultraviolet (UV) cross-linking experiment and an electrophoretic mobility shift assay (EMSA), and demonstrated that the recombinant OlPC4 can be substituted for native OlPC4 in medaka fish intestinal nuclear extracts. In CACO-2 cells, cotransfection of the OlGC6-luciferase fusion genes with an OlPC4 expression vector resulted in 1.5-fold stimulation of the OlGC6 promoter.
Type: article (author version)
URI: http://hdl.handle.net/2115/14406
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 範男

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