|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Involvement of general transcriptional coactivator PC4 in the transcription of medaka fish intestine-specific membrane guanylyl cyclase gene (OlGC6).
| Title: | Involvement of general transcriptional coactivator PC4 in the transcription of medaka fish intestine-specific membrane guanylyl cyclase gene (OlGC6). |
| Authors: | Nakauchi, Mina Browse this author | | Yoshino, Ken-ichi Browse this author | | Yonezawa, Kazuyoshi Browse this author | | Suzuki, Norio Browse this author |
| Keywords: | coactivator | | intestine-specific membrane guanylyl cyclase | | Medaka fish | | positive cofactor 4, transcription |
| Issue Date: | Apr-2005 |
| Publisher: | Oxford University Press |
| Journal Title: | Journal of Biochemistry |
| Volume: | 137 |
| Issue: | 4 |
| Start Page: | 509 |
| End Page: | 515 |
| Publisher DOI: | 10.1093/jb/mvi066 |
| PMID: | 15858175 |
| Abstract: | A recent study showed that the AGACCTTTGC nucleotides sequence (between –90 and –81) contained in the cis-regulatory element in an intestine-specific membrane guanylyl cyclase gene, OlGC6, of the medaka fish, Oryzias latipes, are important for the transcription of the gene in mammalian cultured cell line and in medaka fish. Using sequence-specific DNA affinity chromatography, we purified a cis-regulatory element–binding protein from a medaka fish intestinal nuclear extract and used mass spectrometry to identify it as a medaka fish homologue of general transcriptional coactivator PC4, which we designated as OlPC4. The expression of the OlPC4 gene was detected in embryos, as well as in a large variety of tissues of adult medaka fish. Using a 17-kDa recombinant OlPC4, we carried out an ultraviolet (UV) cross-linking experiment and an electrophoretic mobility shift assay (EMSA), and demonstrated that the recombinant OlPC4 can be substituted for native OlPC4 in medaka fish intestinal nuclear extracts. In CACO-2 cells, cotransfection of the OlGC6-luciferase fusion genes with an OlPC4 expression vector resulted in 1.5-fold stimulation of the OlGC6 promoter. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/14406 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 鈴木 範男
|
