|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Information Science and Technology / Faculty of Information Science and Technology >
Peer-reviewed Journal Articles, etc >
Specificity of hybridization between DNA sequences based on free energy
| Title: | Specificity of hybridization between DNA sequences based on free energy |
| Authors: | Tanaka, F.1 Browse this author | | Kameda, A. Browse this author | | Yamamoto, M. Browse this author →KAKEN DB | | Ohuchi, A. Browse this author |
| Authors(alt): | 田中, 文昭1 |
| Issue Date: | 2006 |
| Publisher: | Springer |
| Journal Title: | DNA COMPUTING |
| Volume: | 3892 |
| Start Page: | 371 |
| End Page: | 379 |
| Abstract: | We investigated the specificity of hybridization based on a minimum free energy (Delta G(min)) through gel electrophoresis analysis. The analysis, using 94 pairs of sequences with length 20, showed that sequences that hybridize each other can be separated using the constraint Delta G(min) < -14.0, but cannot be separated using the number of base pairs (BP) in the range from 9 to 18. This demonstrates that the Delta G(min) is superior to the BP in terms of the capability to separate specific from non-specific sequences. Furthermore, the comparison between sequence design based on Delta G(min) and that based on the BP, done through a computer simulation, showed that the former outperformed the latter in terms of the number of sequences designed successfully as well as the ratio of successfully designed sequences to the total number of sequences checked. |
| Rights: | The original publication is available at www.springerlink.com |
| Relation: | http://www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/14471 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 田中 文昭
|
