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Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) Gene of Arabidopsis Causes Weak rcd1 Phenotype with Compromised Oxidative-Stress Responses

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タイトル: Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) Gene of Arabidopsis Causes Weak rcd1 Phenotype with Compromised Oxidative-Stress Responses
著者: Fujibe, Takahiro 著作を一覧する
Saji, Hikaru 著作を一覧する
Watahiki, Masaaki K. 著作を一覧する
Yamamoto, Kotaro T. 著作を一覧する
キーワード: Arabidopsis thaliana
methyl viologen (paraquat)
ozone
RCD1
subnuclear localization
発行日: 2006年 8月
出版者: Japan Society for Bioscience, Biotechnology, and Agrochemistry
誌名: Bioscience Biotechnology and Biochemistry
巻: 70
号: 8
開始ページ: 1827
終了ページ: 1831
出版社 DOI: 10.1271/bbb.50673
抄録: rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mutation. Since overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. The transgenic lines exhibited a weak rcd1-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant rcd1-2 phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region.
Relation (URI): http://www.jstage.jst.go.jp/
資料タイプ: article
URI: http://hdl.handle.net/2115/14727
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 山本 興太朗

 

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