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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) Gene of Arabidopsis Causes Weak rcd1 Phenotype with Compromised Oxidative-Stress Responses
| Title: | Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) Gene of Arabidopsis Causes Weak rcd1 Phenotype with Compromised Oxidative-Stress Responses |
| Authors: | Fujibe, Takahiro Browse this author | | Saji, Hikaru Browse this author | | Watahiki, Masaaki K. Browse this author →KAKEN DB | | Yamamoto, Kotaro T. Browse this author →KAKEN DB |
| Keywords: | Arabidopsis thaliana | | methyl viologen (paraquat) | | ozone | | RCD1 | | subnuclear localization |
| Issue Date: | Aug-2006 |
| Publisher: | Japan Society for Bioscience, Biotechnology, and Agrochemistry |
| Journal Title: | Bioscience Biotechnology and Biochemistry |
| Volume: | 70 |
| Issue: | 8 |
| Start Page: | 1827 |
| End Page: | 1831 |
| Publisher DOI: | 10.1271/bbb.50673 |
| PMID: | 16926493 |
| Abstract: | rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mutation. Since overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. The transgenic lines exhibited a weak rcd1-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant rcd1-2 phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region. |
| Relation: | http://www.jstage.jst.go.jp/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/14727 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山本 興太朗
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