HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
薬学研究院  >
雑誌発表論文等  >

Tyr-199 and charged residues of pharaonis Phoborhodopsin are important for the interaction with its transducer.

フルテキスト
BJ83-1.pdf158.43 kBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/16002

タイトル: Tyr-199 and charged residues of pharaonis Phoborhodopsin are important for the interaction with its transducer.
著者: Sudo, Yuki 著作を一覧する
Iwamoto, Masayuki 著作を一覧する
Shimono, Kazumi 著作を一覧する
Kamo, Naoki 著作を一覧する
キーワード: Sensory rhodopsin
protein-protein interaction
Mintermediate
Halobacterium salinarum
Natronobacterium pharaonis
発行日: 2002年 7月
出版者: The Biophysical Society
誌名: Biophysical Journal
巻: 83
号: 1
開始ページ: 427
終了ページ: 432
抄録: pharaonis Phoborhodopsin (ppR; also pharaonis sensory rhodopsin II, psRII) is a retinal protein in Natronobacterium pharaonis and is a receptor of negative phototaxis. It forms a complex with its transducer, pHtrII, in membranes and transmits light signals by protein-protein interaction. Tyr-199 is conserved completely in phoborhodopsins among a variety of archaea, but it is replaced by Val (for bacteriorhodopsin) and Phe (for sensory rhodopsin I). Previously, we (Sudo, Y., M. Iwamoto, K. Shimono, and N. Kamo, submitted for publication) showed that analysis of flash-photolysis data of a complex between D75N and the truncated pHtrII (t-Htr) give a good estimate of the dissociation constant KD in the dark. To investigate the importance of Tyr-199, KD of double mutants of D75N/Y199F or D75N/Y199V with t-Htr was estimated by flash-photolysis and was ~10-fold larger than that of D75N, showing the significant contribution of Tyr-199 to binding. The KD of the D75N/t-Htr complex increased with decreasing pH, and the data fitted well with the Henderson-Hasselbach equation with a single pKa of 3.86 ± 0.02. This suggests that certain deprotonated carboxyls at the surface of the transducer (possibly Asp-102, Asp-104, and Asp-106) are needed for the binding.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/16002
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 加茂 直樹

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学