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The elution profile of immobilized liposome chromatography: determination of association and dissociation rate constants.
| Title: | The elution profile of immobilized liposome chromatography: determination of association and dissociation rate constants. |
| Authors: | Ohno, Masako Browse this author | | Ikehara, Tatsuya Browse this author | | Nara, Toshifumi Browse this author | | Kamo, Naoki Browse this author | | Miyauchi, Seiji Browse this author |
| Keywords: | Plate height | | Phosphonium cation | | Retention time | | Band-broadening | | Rate constant |
| Issue Date: | 11-Oct-2004 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Biochimica et Biophysica Acta (BBA) - Biomembranes |
| Volume: | 1665 |
| Issue: | 1-2 |
| Start Page: | 167 |
| End Page: | 176 |
| Publisher DOI: | 10.1016/j.bbamem.2004.07.007 |
| PMID: | 15471582 |
| Abstract: | The interaction of lipophilic cations, tetraphenylphosphonium and triphenylphosphonium homologues with liposomes was investigated using immobilized liposome chromatography (ILC). Large unilamellar liposomes with a mean diameter of 100 nm were stably immobilized in chromatographic gel beads by avidin–biotin. The distribution coefficient calculated from (Ve−V0)/Vs (Ve, retention volume; V0, the void volume; Vs, the stationary phase volume) was found to be independent of flow rate, injection amount and gel bed volume, which is consistent with chromatograph theory. The relationship between the bandwidth and solvent flow rate did not follow band-broadening theories reported thus far. We hypothesized that the solvent might be forced to produce large eddies, spirals or turbulent flow due to the presence of liposomes fixed in the gel. Therefore, we developed a new theory for ILC elution: The column is composed of a number of thin disks containing liposomes and solution, and within each disk the solution is well mixed. This theory accounts for our results, and we were able to use it to estimate the rate constants of association and dissociation of the phosphonium to/from liposomes. |
| Relation: | http://www.sciencedirect.com/science/journal/00052736 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/16003 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 加茂 直樹
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