HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
薬学研究院  >
雑誌発表論文等  >

The elution profile of immobilized liposome chromatography: determination of association and dissociation rate constants.

フルテキスト
BBAB1665-1-2.pdf239.29 kBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/16003

タイトル: The elution profile of immobilized liposome chromatography: determination of association and dissociation rate constants.
著者: Ohno, Masako 著作を一覧する
Ikehara, Tatsuya 著作を一覧する
Nara, Toshifumi 著作を一覧する
Kamo, Naoki 著作を一覧する
Miyauchi, Seiji 著作を一覧する
キーワード: Plate height
Phosphonium cation
Retention time
Band-broadening
Rate constant
発行日: 2004年10月11日
出版者: Elsevier B.V.
誌名: Biochimica et Biophysica Acta (BBA) - Biomembranes
巻: 1665
号: 1-2
開始ページ: 167
終了ページ: 176
出版社 DOI: 10.1016/j.bbamem.2004.07.007
抄録: The interaction of lipophilic cations, tetraphenylphosphonium and triphenylphosphonium homologues with liposomes was investigated using immobilized liposome chromatography (ILC). Large unilamellar liposomes with a mean diameter of 100 nm were stably immobilized in chromatographic gel beads by avidin–biotin. The distribution coefficient calculated from (Ve−V0)/Vs (Ve, retention volume; V0, the void volume; Vs, the stationary phase volume) was found to be independent of flow rate, injection amount and gel bed volume, which is consistent with chromatograph theory. The relationship between the bandwidth and solvent flow rate did not follow band-broadening theories reported thus far. We hypothesized that the solvent might be forced to produce large eddies, spirals or turbulent flow due to the presence of liposomes fixed in the gel. Therefore, we developed a new theory for ILC elution: The column is composed of a number of thin disks containing liposomes and solution, and within each disk the solution is well mixed. This theory accounts for our results, and we were able to use it to estimate the rate constants of association and dissociation of the phosphonium to/from liposomes.
Relation (URI): http://www.sciencedirect.com/science/journal/00052736
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/16003
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 加茂 直樹

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学