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Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.

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Title: Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.
Authors: Sudo, Yuki Browse this author
Iwamoto, Masayuki Browse this author
Shimono, Kazumi Browse this author
Kamo, Naoki Browse this author
Keywords: Sensory rhodopsin
Protein–protein interaction
Photocycle
Flash-photolysis
M-intermediate
Halobacterium salinarum
Issue Date: Jul-2002
Publisher: Elsevier Science B.V.
Journal Title: Journal of Photochemistry and Photobiology B Biology
Volume: 67
Issue: 3
Start Page: 171
End Page: 176
Publisher DOI: 10.1016/S1011-1344(02)00322-6
PMID: 12167316
Abstract: Pharaonis phoborhodopsin (ppR or pharaonis sensory rhodopsin II) is a receptor of the negative phototaxis of Natronobacterium pharaonis and forms a complex with its transducer pHtrII in membranes. Flash-photolyis of a D75N mutant did not yield the M-intermediate, but an O-like intermediate is observed in a ms time range. We examined the interaction between the D75N of ppR and t-Htr (truncated pHtrII). These formed a complex in the presence of 0.1% n-dodecyl-β-maltoside, and the association accelerated the decay of the O of D75N from 15 to 56 s−1. From the decay time constants under varying ratios of D75N and t-Htr, n, the molar ratio of D75N/t-Htr in the complex, and KD, the dissociation constant, were estimated. The value of n was unity and KD was estimated to 146 nM. This KD value can be considered to be the association between the photo-intermediate and t-Htr, which is deduced by the method of estimation. Previously we (Photochem. Photobiol. 74 (2001) 489) reported a KD of 15 μM for the interaction between the wild-type and t-Htr by means of the change in M-decay rates. Therefore, this value should be the KD value for the interaction between M of the wild-type and t-Htr.
Relation: http://www.sciencedirect.com/science/journal/10111344
Type: article (author version)
URI: http://hdl.handle.net/2115/16004
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加茂 直樹

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