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Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.

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タイトル: Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.
著者: Sudo, Yuki 著作を一覧する
Iwamoto, Masayuki 著作を一覧する
Shimono, Kazumi 著作を一覧する
Kamo, Naoki 著作を一覧する
キーワード: Sensory rhodopsin
Protein–protein interaction
Photocycle
Flash-photolysis
M-intermediate
Halobacterium salinarum
発行日: 2002年 7月
出版者: Elsevier Science B.V.
誌名: Journal of Photochemistry and Photobiology B Biology
巻: 67
号: 3
開始ページ: 171
終了ページ: 176
出版社 DOI: 10.1016/S1011-1344(02)00322-6
抄録: Pharaonis phoborhodopsin (ppR or pharaonis sensory rhodopsin II) is a receptor of the negative phototaxis of Natronobacterium pharaonis and forms a complex with its transducer pHtrII in membranes. Flash-photolyis of a D75N mutant did not yield the M-intermediate, but an O-like intermediate is observed in a ms time range. We examined the interaction between the D75N of ppR and t-Htr (truncated pHtrII). These formed a complex in the presence of 0.1% n-dodecyl-β-maltoside, and the association accelerated the decay of the O of D75N from 15 to 56 s−1. From the decay time constants under varying ratios of D75N and t-Htr, n, the molar ratio of D75N/t-Htr in the complex, and KD, the dissociation constant, were estimated. The value of n was unity and KD was estimated to 146 nM. This KD value can be considered to be the association between the photo-intermediate and t-Htr, which is deduced by the method of estimation. Previously we (Photochem. Photobiol. 74 (2001) 489) reported a KD of 15 μM for the interaction between the wild-type and t-Htr by means of the change in M-decay rates. Therefore, this value should be the KD value for the interaction between M of the wild-type and t-Htr.
Relation (URI): http://www.sciencedirect.com/science/journal/10111344
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/16004
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 加茂 直樹

 

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