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Association of pharaonis phoborhodopsin with its cognate transducer decreases the photo-dependent reactivity by water-soluble reagents of azide and hydroxylamine.
| Title: | Association of pharaonis phoborhodopsin with its cognate transducer decreases the photo-dependent reactivity by water-soluble reagents of azide and hydroxylamine. |
| Authors: | Sudo, Yuki Browse this author | | Iwamoto, Masayuki Browse this author | | Shimono, Kazumi Browse this author | | Kamo, Naoki Browse this author |
| Keywords: | Bleach of retinoid protein | | Flash photolysis | | Sensory rhodopsin | | Halobacterium salinarum | | Natronobacterium pharaonis |
| Issue Date: | 2-Jan-2002 |
| Publisher: | Elsevier Science B.V. |
| Journal Title: | Biochimica et Biophysica Acta (BBA) - Biomembranes |
| Volume: | 1558 |
| Issue: | 1 |
| Start Page: | 63 |
| End Page: | 69 |
| Publisher DOI: | 10.1016/S0005-2736(01)00423-0 |
| PMID: | 11750265 |
| Abstract: | pharaonis phoborhodopsin (ppR; also pharaonis sensory rhodopsin II, psRII) is a receptor of the negative phototaxis of Natronobacterium pharaonis. In halobacterial membrane, ppR forms a complex with its transducer pHtrII, and this complex transmits the light signal to the sensory system in the cytoplasm. In the present work, the truncated transducer, t-Htr, was used which interacts with ppR [Sudo et al. (2001) Photochem. Photobiol. 74, 489–494]. Two water-soluble reagents, hydroxylamine and azide, reacted both with the transducer-free ppR and with the complex ppR/t-Htr (the complex between ppR and its truncated transducer). In the dark, the bleaching rates caused by hydroxylamine were not significantly changed between transducer-free ppR and ppR/t-Htr, or that of the free ppR was a little slower. Illumination accelerated the bleach rates, which is consistent with our previous conclusion that the reaction occurs selectively at the M-intermediate, but the rate of the complex was about 7.4-fold slower than that of the transducer-free ppR. Azide accelerated the M-decay, and its reaction rate of ppR/t-Htr was about 4.6-fold slower than free ppR. These findings suggest that the transducer binding decreases the water accessibility around the chromophore at the M-intermediate. Its implication is discussed. |
| Relation: | http://www.sciencedirect.com/science/journal/00052736 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/16005 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 加茂 直樹
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