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Association of pharaonis phoborhodopsin with its cognate transducer decreases the photo-dependent reactivity by water-soluble reagents of azide and hydroxylamine.

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Title: Association of pharaonis phoborhodopsin with its cognate transducer decreases the photo-dependent reactivity by water-soluble reagents of azide and hydroxylamine.
Authors: Sudo, Yuki Browse this author
Iwamoto, Masayuki Browse this author
Shimono, Kazumi Browse this author
Kamo, Naoki Browse this author
Keywords: Bleach of retinoid protein
Flash photolysis
Sensory rhodopsin
Halobacterium salinarum
Natronobacterium pharaonis
Issue Date: 2-Jan-2002
Publisher: Elsevier Science B.V.
Journal Title: Biochimica et Biophysica Acta (BBA) - Biomembranes
Volume: 1558
Issue: 1
Start Page: 63
End Page: 69
Publisher DOI: 10.1016/S0005-2736(01)00423-0
PMID: 11750265
Abstract: pharaonis phoborhodopsin (ppR; also pharaonis sensory rhodopsin II, psRII) is a receptor of the negative phototaxis of Natronobacterium pharaonis. In halobacterial membrane, ppR forms a complex with its transducer pHtrII, and this complex transmits the light signal to the sensory system in the cytoplasm. In the present work, the truncated transducer, t-Htr, was used which interacts with ppR [Sudo et al. (2001) Photochem. Photobiol. 74, 489–494]. Two water-soluble reagents, hydroxylamine and azide, reacted both with the transducer-free ppR and with the complex ppR/t-Htr (the complex between ppR and its truncated transducer). In the dark, the bleaching rates caused by hydroxylamine were not significantly changed between transducer-free ppR and ppR/t-Htr, or that of the free ppR was a little slower. Illumination accelerated the bleach rates, which is consistent with our previous conclusion that the reaction occurs selectively at the M-intermediate, but the rate of the complex was about 7.4-fold slower than that of the transducer-free ppR. Azide accelerated the M-decay, and its reaction rate of ppR/t-Htr was about 4.6-fold slower than free ppR. These findings suggest that the transducer binding decreases the water accessibility around the chromophore at the M-intermediate. Its implication is discussed.
Relation: http://www.sciencedirect.com/science/journal/00052736
Type: article (author version)
URI: http://hdl.handle.net/2115/16005
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加茂 直樹

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