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Preparation and Properties of Immobilized Mercuric Reductase in Porous Glass Carriers

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タイトル: Preparation and Properties of Immobilized Mercuric Reductase in Porous Glass Carriers
その他のタイトル: 多孔質ガラス担体に固定化した水銀還元酵素の作製及びその特性
著者: UO, Motohiro 著作を一覧する
NUMATA, Masahiko 著作を一覧する
SUZUKI, Masayasu 著作を一覧する
TAMIYA, Eiichi 著作を一覧する
KARUBE, Isao 著作を一覧する
MAKISHIMA, Akio 著作を一覧する
キーワード: Mercuric reductase
Porous glass
Immobilized enzyme
pH profile
Mercuric ion sensor
発行日: 1992年 4月
出版者: 社団法人 日本セラミックス協会(The Ceramic Society of Japan)
誌名: Journal of Ceramic Society of Japan
巻: 100
開始ページ: 430
終了ページ: 433
抄録: The immobilized mercuric reductase covalently coupled to porous glass was prepared and its properties were investigated. Mercuric reductase immobilized on arylamino and carboxyl derivatives exhibited high relative activities about 20-30% compared with soluble enzymes. The optimum pH of mercuric reductase immobilized on these derivatives was 7.5, slightly higher than that of soluble state (7.0-7.2). The stability of mercuric reductase increased with immobilization onto arylamino derivative and the immobilized enzyme maintained at 30℃ for 29 days showed 80-90% of initial activity. As the application of immobilized enzyme, mercuric ion sensor was prepared and it was found that mercuric ion higher than 0.5μM was detectable with this sensor.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 宇尾 基弘


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