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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
Preparation and Properties of Immobilized Mercuric Reductase in Porous Glass Carriers
| Title: | Preparation and Properties of Immobilized Mercuric Reductase in Porous Glass Carriers |
| Other Titles: | 多孔質ガラス担体に固定化した水銀還元酵素の作製及びその特性 |
| Authors: | UO, Motohiro1 Browse this author →KAKEN DB | | NUMATA, Masahiko2 Browse this author | | SUZUKI, Masayasu3 Browse this author | | TAMIYA, Eiichi4 Browse this author | | KARUBE, Isao5 Browse this author | | MAKISHIMA, Akio6 Browse this author |
| Authors(alt): | 宇尾, 基弘1 | | 沼田, 雅彦2 | | 鈴木, 正康3 | | 民谷, 栄一4 | | 軽部, 征夫5 | | 牧島, 亮男6 |
| Keywords: | Mercuric reductase | | Porous glass | | Immobilized enzyme | | Carrier | | Activity | | pH profile | | Stability | | Mercuric ion sensor |
| Issue Date: | Apr-1992 |
| Publisher: | 社団法人 日本セラミックス協会 |
| Journal Title: | Journal of Ceramic Society of Japan |
| Volume: | 100 |
| Start Page: | 430 |
| End Page: | 433 |
| Abstract: | The immobilized mercuric reductase covalently coupled to porous glass was prepared and its properties were investigated. Mercuric reductase immobilized on arylamino and carboxyl derivatives exhibited high relative activities about 20-30% compared with soluble enzymes. The optimum pH of mercuric reductase immobilized on these derivatives was 7.5, slightly higher than that of soluble state (7.0-7.2). The stability of mercuric reductase increased with immobilization onto arylamino derivative and the immobilized enzyme maintained at 30℃ for 29 days showed 80-90% of initial activity. As the application of immobilized enzyme, mercuric ion sensor was prepared and it was found that mercuric ion higher than 0.5μM was detectable with this sensor. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/17009 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宇尾 基弘
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