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Preparation and Properties of Immobilized Mercuric Reductase in Porous Glass Carriers

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Title: Preparation and Properties of Immobilized Mercuric Reductase in Porous Glass Carriers
Other Titles: 多孔質ガラス担体に固定化した水銀還元酵素の作製及びその特性
Authors: UO, Motohiro1 Browse this author →KAKEN DB
NUMATA, Masahiko2 Browse this author
SUZUKI, Masayasu3 Browse this author
TAMIYA, Eiichi4 Browse this author
KARUBE, Isao5 Browse this author
MAKISHIMA, Akio6 Browse this author
Authors(alt): 宇尾, 基弘1
沼田, 雅彦2
鈴木, 正康3
民谷, 栄一4
軽部, 征夫5
牧島, 亮男6
Keywords: Mercuric reductase
Porous glass
Immobilized enzyme
pH profile
Mercuric ion sensor
Issue Date: Apr-1992
Publisher: 社団法人 日本セラミックス協会
Journal Title: Journal of Ceramic Society of Japan
Volume: 100
Start Page: 430
End Page: 433
Abstract: The immobilized mercuric reductase covalently coupled to porous glass was prepared and its properties were investigated. Mercuric reductase immobilized on arylamino and carboxyl derivatives exhibited high relative activities about 20-30% compared with soluble enzymes. The optimum pH of mercuric reductase immobilized on these derivatives was 7.5, slightly higher than that of soluble state (7.0-7.2). The stability of mercuric reductase increased with immobilization onto arylamino derivative and the immobilized enzyme maintained at 30℃ for 29 days showed 80-90% of initial activity. As the application of immobilized enzyme, mercuric ion sensor was prepared and it was found that mercuric ion higher than 0.5μM was detectable with this sensor.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宇尾 基弘

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