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Temporal and spatial variations of lipid droplets during adipocyte division and differentiation

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Title: Temporal and spatial variations of lipid droplets during adipocyte division and differentiation
Authors: Nagayama, Masafumi Browse this author →KAKEN DB
Uchida, Tsutomu Browse this author →KAKEN DB
Gohara, Kazutoshi Browse this author →KAKEN DB
Keywords: time-lapse observation
fluorescence observation
live cell imaging
visceral adipocyte
adipose conversion
lipid bodies
lipid droplet-associated proteins
Issue Date: Jan-2007
Publisher: American Society for Biochemistry and Molecular Biology
Journal Title: The Journal of Lipid Research
Volume: 48
Issue: 1
Start Page: 9
End Page: 18
Publisher DOI: 10.1194/jlr.M600155-JLR200
PMID: 17057226
Abstract: By capturing time-lapse images of primary stromal-vascular cells (SVCs) derived from rat mesenteric adipose tissue, we revealed temporal and spatial variations of lipid droplets (LDs) in individual SVCs during adipocyte differentiation. Numerous small LDs (a few micrometers in diameter) appeared in the perinuclear region at an early stage of differentiation; subsequently, several LDs grew to more than 10 µm in diameter and occupied the cytoplasm. We have developed a method for the fluorescence staining of LDs in living adipocytes. Time-lapse observation of the stained cells at higher magnification showed that nascent LDs (several 100 nm in diameter) grew into small LDs while moving from lamellipodia to the perinuclear region. We also found that adipocytes are capable of division and that they evenly distribute the LDs between two daughter cells. Immunofluorescence observations of LD-associated proteins revealed that such cell divisions of SVCs occurred even after LDs were coated with perilipin, suggesting that the "final" cell division during adipocyte differentiation occurs considerably later than that characterized in 3T3-L1 cells. Our time-lapse observations have provided a detailed account of the morphological changes that SVCs undergo during adipocyte division and differentiation.
Rights: Copyright © 2007 by the American Society for Biochemistry and Molecular
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永山 昌史

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