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Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans.

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Title: Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans.
Authors: Shimada, Masumi Browse this author
Kanematsu, Kenji Browse this author
Tanaka, Keiji Browse this author →KAKEN DB
Yokosawa, Hideyoshi Browse this author
Kawahara, Hiroyuki Browse this author
Issue Date: Dec-2006
Publisher: The American Society for Cell Biology
Journal Title: Molecular Biology of the Cell
Volume: 17
Issue: 12
Start Page: 5356
End Page: 5371
Publisher DOI: 10.1091/mbc.E06-05-0437
PMID: 17050737
Abstract: The ubiquitin-binding RPN-10 protein serves as a ubiquitin receptor that delivers client proteins to the 26S proteasome. Although ubiquitin recognition is an essential step for proteasomal destruction, deletion of the rpn-10 gene in yeast does not influence viability, indicating redundancy of the substrate delivery pathway. However, their specificity and biological relevance in higher eukaryotes is still enigmatic. We report herein that knockdown of the rpn-10 gene, but not any other proteasome subunit genes, sexually transforms hermaphrodites to females by eliminating hermaphrodite spermatogenesis in Caenorhabditis elegans. The feminization phenotype induced by deletion of the rpn-10 gene was rescued by knockdown of tra-2, one of sexual fate decision genes promoting female development, and its downstream target tra-1, indicating that the TRA-2–mediated sex determination pathway is crucial for the rpn-10–induced sterile phenotype. Intriguingly, we found that co-knockdown of Δrpn-10 and functionally related ubiquitin ligase ufd-2 overcomes the germline-musculinizing effect of fem-3(gf). Furthermore, TRA-2 proteins accumulated in rpn-10-defective worms. Our results show that the RPN-10–mediated ubiquitin pathway is indispensable for control of the TRA-2–mediated sex-determining pathway.
Rights: © 2006 by The American Society for Cell Biology
Type: article
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 裕之

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