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Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans.

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タイトル: Proteasomal ubiquitin receptor RPN-10 controls sex determination in Caenorhabditis elegans.
著者: Shimada, Masumi 著作を一覧する
Kanematsu, Kenji 著作を一覧する
Tanaka, Keiji 著作を一覧する
Yokosawa, Hideyoshi 著作を一覧する
Kawahara, Hiroyuki 著作を一覧する
発行日: 2006年12月
出版者: The American Society for Cell Biology
誌名: Molecular Biology of the Cell
巻: 17
号: 12
開始ページ: 5356
終了ページ: 5371
出版社 DOI: 10.1091/mbc.E06-05-0437
抄録: The ubiquitin-binding RPN-10 protein serves as a ubiquitin receptor that delivers client proteins to the 26S proteasome. Although ubiquitin recognition is an essential step for proteasomal destruction, deletion of the rpn-10 gene in yeast does not influence viability, indicating redundancy of the substrate delivery pathway. However, their specificity and biological relevance in higher eukaryotes is still enigmatic. We report herein that knockdown of the rpn-10 gene, but not any other proteasome subunit genes, sexually transforms hermaphrodites to females by eliminating hermaphrodite spermatogenesis in Caenorhabditis elegans. The feminization phenotype induced by deletion of the rpn-10 gene was rescued by knockdown of tra-2, one of sexual fate decision genes promoting female development, and its downstream target tra-1, indicating that the TRA-2–mediated sex determination pathway is crucial for the rpn-10–induced sterile phenotype. Intriguingly, we found that co-knockdown of Δrpn-10 and functionally related ubiquitin ligase ufd-2 overcomes the germline-musculinizing effect of fem-3(gf). Furthermore, TRA-2 proteins accumulated in rpn-10-defective worms. Our results show that the RPN-10–mediated ubiquitin pathway is indispensable for control of the TRA-2–mediated sex-determining pathway.
Rights: © 2006 by The American Society for Cell Biology
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 川原 裕之


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