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Characterization of mouse tissue kallikrein 5

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タイトル: Characterization of mouse tissue kallikrein 5
著者: Rajapakse, Sanath 著作を一覧する
Ogiwara, Katsueki 著作を一覧する
Yamano, Noriko 著作を一覧する
Kimura, Atsushi 著作を一覧する
Hirata, Kensaku 著作を一覧する
Takahashi, Sumio 著作を一覧する
Takahashi, Takayuki 著作を一覧する
キーワード: mouse
kallikrein 5
recombinant enzyme
発行日: 2006年11月
出版者: Zoological Society of Japan
巻: 23
号: 11
開始ページ: 963
終了ページ: 968
出版社 DOI: 10.2108/zsj.23.963
抄録: Mouse tissue kallikreins (Klks) are members of a large, multigene family consisting of 37 genes, 26 of which can code for functional proteins. Mouse tissue kallikrein 5 (Klk5) has long been thought to be one of these functional genes, but the gene product, mK5, has not been isolated and characterized. In the present study, we prepared active recombinant mK5 using an Escherichia coli expression system, followed by column chromatography. We then determined the biochemical and enzymatic properties of purified mK5. mK5 had trypsin-like activity for Arg at the P1 position, and its activity was inhibited by typical serine protease inhibitors. mK5 degraded gelatin, fibronectin, collagen type IV, high-molecular-weight kininogen, and insulin-like growth factor binding protein-3. Our data suggest that mK5 may be implicated in the process of extracellular matrix remodeling.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高橋 孝行


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