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Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells
| Title: | Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells |
| Authors: | Hoshino, Yoichiro Browse this author →KAKEN DB | | Scholten, Stefan Browse this author | | von Wiegen, Petra Browse this author | | Lörz, Horst Browse this author | | Kranz, Erhard Browse this author |
| Keywords: | Anti-α-tubulin antibody | | Maize | | Microtubule | | Zea mays | | Zygote development |
| Issue Date: | Jul-2004 |
| Publisher: | Springer |
| Journal Title: | Sexual Plant Reproduction |
| Volume: | 17 |
| Issue: | 2 |
| Start Page: | 89 |
| End Page: | 95 |
| Publisher DOI: | 10.1007/s00497-004-0221-0 |
| Abstract: | By using single cell micromanipulation techniques, we developed an immunocytochemical procedure to examine subcellular protein localization in isolated and cultured cells. Localization of microtubules was examined in isolated single egg cells and developing zygotes of maize with anti--tubulin antibodies. In egg cells, a few cortical microtubules were detected but well organized microtubules were rarely observed. In contrast, distinct cortical microtubules and strands of cytoplasmic microtubules radiating from the nucleus to the cell periphery were observed in developing zygotes. Solely cortical microtubules were observed in zygotes up to 7 h after in vitro fertilization. After this time, radiating microtubules additionally appeared, and persisted during zygote development. These results indicate early and pronounced fertilization-induced changes in microtubular organization in the fertilized egg cell of maize. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/18874 |
| Appears in Collections: | 北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 星野 洋一郎
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