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Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells

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タイトル: Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells
著者: Hoshino, Yoichiro 著作を一覧する
Scholten, Stefan 著作を一覧する
von Wiegen, Petra 著作を一覧する
Lörz, Horst 著作を一覧する
Kranz, Erhard 著作を一覧する
キーワード: Anti-α-tubulin antibody
Maize
Microtubule
Zea mays
Zygote development
発行日: 2004年 7月
出版者: Springer
誌名: Sexual Plant Reproduction
巻: 17
号: 2
開始ページ: 89
終了ページ: 95
出版社 DOI: 10.1007/s00497-004-0221-0
抄録: By using single cell micromanipulation techniques, we developed an immunocytochemical procedure to examine subcellular protein localization in isolated and cultured cells. Localization of microtubules was examined in isolated single egg cells and developing zygotes of maize with anti--tubulin antibodies. In egg cells, a few cortical microtubules were detected but well organized microtubules were rarely observed. In contrast, distinct cortical microtubules and strands of cytoplasmic microtubules radiating from the nucleus to the cell periphery were observed in developing zygotes. Solely cortical microtubules were observed in zygotes up to 7 h after in vitro fertilization. After this time, radiating microtubules additionally appeared, and persisted during zygote development. These results indicate early and pronounced fertilization-induced changes in microtubular organization in the fertilized egg cell of maize.
Rights: The original publication is available at www.springerlink.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/18874
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 星野 洋一郎

 

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