HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Field Science Center for Northern Biosphere >
Peer-reviewed Journal Articles, etc >

Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells

Files in This Item:
SPR17-2.pdf194.5 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/18874

Title: Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote—an immunocytochemical approach adapted to single cells
Authors: Hoshino, Yoichiro Browse this author →KAKEN DB
Scholten, Stefan Browse this author
von Wiegen, Petra Browse this author
Lörz, Horst Browse this author
Kranz, Erhard Browse this author
Keywords: Anti-α-tubulin antibody
Maize
Microtubule
Zea mays
Zygote development
Issue Date: Jul-2004
Publisher: Springer
Journal Title: Sexual Plant Reproduction
Volume: 17
Issue: 2
Start Page: 89
End Page: 95
Publisher DOI: 10.1007/s00497-004-0221-0
Abstract: By using single cell micromanipulation techniques, we developed an immunocytochemical procedure to examine subcellular protein localization in isolated and cultured cells. Localization of microtubules was examined in isolated single egg cells and developing zygotes of maize with anti--tubulin antibodies. In egg cells, a few cortical microtubules were detected but well organized microtubules were rarely observed. In contrast, distinct cortical microtubules and strands of cytoplasmic microtubules radiating from the nucleus to the cell periphery were observed in developing zygotes. Solely cortical microtubules were observed in zygotes up to 7 h after in vitro fertilization. After this time, radiating microtubules additionally appeared, and persisted during zygote development. These results indicate early and pronounced fertilization-induced changes in microtubular organization in the fertilized egg cell of maize.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/18874
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 星野 洋一郎

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University