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Silencing of Exogenous DNA in Cultured Cells

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Title: Silencing of Exogenous DNA in Cultured Cells
Authors: Ochiai, Hiroshi Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Kamiya, Hiroyuki Browse this author
Keywords: exogenous DNA
intranuclear disposition
Issue Date: Jun-2006
Publisher: Pharmaceutical Society of Japan
Journal Title: Biological & Pharmaceutical Bulletin
Volume: 29
Issue: 6
Start Page: 1294
End Page: 1296
Publisher DOI: 10.1248/bpb.29.1294
Abstract: The intranuclear disposition of exogenous DNA is highly important for the therapeutic effects of the administrated DNA. Naked luciferase-plasmid DNA was transfected into cultured cells including HeLa by electroporation, and the amounts of intranuclear plasmid DNA and luciferase were quantitated at various time points. Decrease in expression efficiency from one copy of the exogenous DNA over time occurred as the case of mouse liver, and its degrees varied between cell lines. These results suggest that exogenous DNA is 'silenced' in the cultured cells as well as in mouse hepatocytes.
Type: article
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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