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DNA Microarray Analysis of Type 2 Diabetes-Related Genes Co-regulated between White Blood Cells and Livers of Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats

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タイトル: DNA Microarray Analysis of Type 2 Diabetes-Related Genes Co-regulated between White Blood Cells and Livers of Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
著者: Hayashi, Yasuhiro 著作を一覧する
Iida, Shinya 著作を一覧する
Sato, Yuichiro 著作を一覧する
Nakaya, Akihiro 著作を一覧する
Sawada, Akihiro 著作を一覧する
Kaji, Noritada 著作を一覧する
Kamiya, Hiroyuki 著作を一覧する
Baba, Yoshinobu 著作を一覧する
Harashima, Hideyoshi 著作を一覧する
キーワード: white blood cell
type 2 diabetes mellitus
gene expression
oligo DNA microarray
Otsuka Long-Evans Tokushima Fatty rat
発行日: 2007年 4月
出版者: Pharmaceutical Society of Japan
誌名: Biological & Pharmaceutical Bulletin
巻: 30
号: 4
開始ページ: 763
終了ページ: 771
出版社 DOI: 10.1248/bpb.30.763
抄録: In a previous study, we hypothesized that some type 2 diabetes mellitus susceptible genes may be up/downregulated in white blood cells (WBC) of Otsuka Long-Evans Tokushima Fatty (OLETF) rats, reflecting their up/down-regulation in major insulin-target tissues such as the liver before the onset of diabetes. We identified 57 potential candidate genes for predicting diabetes. In this study, we examined this hypothesis further by extending the experimental conditions from before the onset (6 weeks) to after the onset (24 weeks) of diabetes that type 2 diabetes mellitus susceptible genes are co-regulated in WBC, reflecting their expression in the liver. Using rat oligo DNA microarrays, we found that 48 genes are up/down-regulated in OLETF rats compared to control Long-Evans Tokushima Otsuka (LETO) rats in WBC and liver under fasting or insulin administration conditions. Twenty nine and 33 genes were up/down-regulated in both WBC and livers, respectively, under fasting and insulin administration conditions, respectively. Eight out of 29 genes in fasting condition and 12 out of 33 genes in insulin administration conditions have been reported to be type 2 diabetes mellitus susceptible genes and the remainder have not been reported to be related to type 2 diabetes mellitus. These results support our hypothesis that the expression levels of type 2 diabetes mellitus related genes in WBC are reflective of those in the liver after the onset of diabetes.
資料タイプ: article
URI: http://hdl.handle.net/2115/20473
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 紙谷 浩之

 

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