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The location of the left-handedly curved DNA sequence affects exogenous DNA expression in vivo

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Title: The location of the left-handedly curved DNA sequence affects exogenous DNA expression in vivo
Authors: Kamiya, Hiroyuki Browse this author
Fukunaga, Satoki Browse this author
Ohyama, Takashi Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: Left-handedly curved DNA
Exogenous DNA
Intranuclear disposition
Hydrodynamics-based injection
Issue Date: 1-May-2007
Publisher: Elsevier
Journal Title: Archives of Biochemistry and Biophysics
Volume: 461
Issue: 1
Start Page: 7
End Page: 12
Publisher DOI: 10.1016/
PMID: 17382286
Abstract: The intranuclear disposition of a plasmid is extremely important for transgene expression. The effects of a left-handedly curved sequence with high histone affinity on plasmid expression were examined in vivo. A naked luciferase-plasmid was delivered into mouse liver by a hydrodynamics-based injection, and the luciferase activities were quantitated at various time points. The location of the left-handedly curved sequence determined the transgene expression, without affecting the amount of intranuclear exogenous DNA. The plasmid containing the curved sequence at the location that results in the exposure of the TATA box out of the nucleosome core showed the highest expression. These results suggest that sequences with high histone affinity could control transgene expression from plasmids in vivo.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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