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Hokkaido University Collection of Scholarly and Academic Papers >
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High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.
| Title: | High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells. |
| Authors: | Takagi, Mutsumi Browse this author →KAKEN DB | | Umetsu, Yousuke Browse this author | | Fujiwara, Masashi Browse this author →KAKEN DB | | Wakitani, Shigeyuki Browse this author |
| Keywords: | cell density | | mesenchymal stem cell | | differentiation | | chondrocyte |
| Issue Date: | Jan-2007 |
| Publisher: | The Society for Biotechnology, Japan |
| Journal Title: | Journal of Bioscience and Bioengineering |
| Volume: | 103 |
| Issue: | 1 |
| Start Page: | 98 |
| End Page: | 100 |
| Publisher DOI: | 10.1263/jbb.103.98 |
| PMID: | 17298908 |
| Abstract: | The effects of the density of human mesenchymal stem cells (MSCs) on their differentioation to chondrocytes in a differentiation medium supplemented with dexamethasone, TGFβ3, and IGF-1 were investigated for the regenerative therapy of cartilage. The increase in the initial density of MSCs from 0.05×104 to 0.9×104 cells/cm2 accelerated the increase in the expression level of aggrecan mRNA during the differentiation culture for 7 d. The conditioned medium harvested at 7 d from the differentiation culture with an initial MSC density of 0.3×104 cells/cm2 accelerated the initial increase in the expression level for 3 d in the differentiation culture with an initial MSC density of 0.3×104 cells/cm2, whereas the conditioned medium harvested at 7 d in the differentiation culture with an initial MSC density of 0.05×104 cells/cm2 did not. The differentiation culture after 14 d with an initial MSC concentration of 0.3×104 cells/cm2 showed an expression level 1.7-fold that in the case of the culture with an initial MSC concentration of 0.05×104 cells/cm2. Thus, a high MSC inoculum density might be appropriate for the rapid differentiation of MSCs to chondrocytes. |
| Relation: | http://www.sbj.or.jp/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/22086 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高木 睦
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