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High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.

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タイトル: High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells.
著者: Takagi, Mutsumi 著作を一覧する
Umetsu, Yousuke 著作を一覧する
Fujiwara, Masashi 著作を一覧する
Wakitani, Shigeyuki 著作を一覧する
キーワード: cell density
mesenchymal stem cell
differentiation
chondrocyte
発行日: 2007年 1月
出版者: The Society for Biotechnology, Japan
誌名: Journal of Bioscience and Bioengineering
巻: 103
号: 1
開始ページ: 98
終了ページ: 100
出版社 DOI: 10.1263/jbb.103.98
抄録: The effects of the density of human mesenchymal stem cells (MSCs) on their differentioation to chondrocytes in a differentiation medium supplemented with dexamethasone, TGFβ3, and IGF-1 were investigated for the regenerative therapy of cartilage. The increase in the initial density of MSCs from 0.05×104 to 0.9×104 cells/cm2 accelerated the increase in the expression level of aggrecan mRNA during the differentiation culture for 7 d. The conditioned medium harvested at 7 d from the differentiation culture with an initial MSC density of 0.3×104 cells/cm2 accelerated the initial increase in the expression level for 3 d in the differentiation culture with an initial MSC density of 0.3×104 cells/cm2, whereas the conditioned medium harvested at 7 d in the differentiation culture with an initial MSC density of 0.05×104 cells/cm2 did not. The differentiation culture after 14 d with an initial MSC concentration of 0.3×104 cells/cm2 showed an expression level 1.7-fold that in the case of the culture with an initial MSC concentration of 0.05×104 cells/cm2. Thus, a high MSC inoculum density might be appropriate for the rapid differentiation of MSCs to chondrocytes.
Relation (URI): http://www.sbj.or.jp/
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/22086
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高木 睦

 

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