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Leukemia inhibitory factor-induced phosphorylation of STAP-2 on tyrosine-250 is involved in its STAT3-enhancing activity.
| Title: | Leukemia inhibitory factor-induced phosphorylation of STAP-2 on tyrosine-250 is involved in its STAT3-enhancing activity. |
| Authors: | Sekine, Yuichi Browse this author | | Tsuji, Satoshi Browse this author | | Ikeda, Osamu Browse this author | | Kakisaka, Michinori Browse this author | | Sugiyama, Kenji Browse this author | | Yoshimura, Akihiko Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | STAP-2 | | LIF | | Phosphorylation | | Adaptor protein | | STAT3 | | Transcription | | Antibody |
| Issue Date: | 4-May-2007 |
| Publisher: | Elsevier Inc. |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 356 |
| Issue: | 2 |
| Start Page: | 517 |
| End Page: | 522 |
| Publisher DOI: | 10.1016/j.bbrc.2007.03.031 |
| PMID: | 17368569 |
| Abstract: | Signal transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein that contains Pleckstrin and Src homology 2 (SH2)-like domains as well as a YXXQ motif in its C-terminal region. Our previous studies revealed that STAP-2 binds to signal transducer and activator of transcription 3 (STAT3) and STAT5, and regulates their signaling pathways. In the present study, we identified tyrosine-250 (Tyr250) in STAP-2 as a major site of phosphorylation by v-src and Jak2, using a phospho-specific antibody against STAP-2 phosphorylated at Tyr250. Mutational analyses revealed that Tyr250 was involved in the STAT3-enhancing activity of STAP-2. We further found that leukemia inhibitory factor (LIF) stimulated STAP-2 Tyr250 phosphorylation in 293T and Hep3B cells. Moreover, endogenous STAP-2 was phosphorylated at Tyr250 following LIF stimulation of murine M1 cell line. Taken together, our findings demonstrate that endogenous STAP-2 is phosphorylated at Tyr250 and that this phosphorylation is involved in its function. |
| Relation: | http://www.sciencedirect.com/science/journal/0006291X |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/22097 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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