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STAP-2 regulates c-Fms/M-CSF receptor signaling in murine macrophage Raw 264.7 cells.
| Title: | STAP-2 regulates c-Fms/M-CSF receptor signaling in murine macrophage Raw 264.7 cells. |
| Authors: | Ikeda, Osamu Browse this author | | Sekine, Yuichi Browse this author | | Kakisaka, Michinori Browse this author | | Tsuji, Satoshi Browse this author | | Muromoto, Ryuta Browse this author | | Ohbayashi, Norihiko Browse this author | | Oritani, Kenji Browse this author | | Yoshimura, Akihiko Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | Macrophage | | c-Fms | | Tyrosine phosphorylation | | Adaptor protein | | Migration | | Signal transduction |
| Issue Date: | 6-Jul-2007 |
| Publisher: | Elsevier Inc. |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 358 |
| Issue: | 3 |
| Start Page: | 931 |
| End Page: | 937 |
| Publisher DOI: | 10.1016/j.bbrc.2007.05.030 |
| PMID: | 17512498 |
| Abstract: | Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein as a c-Fms/M-CSF receptor-interacting protein and constitutively expressed in macrophages. Our previous studies also revealed that STAP-2 binds to MyD88 and IKK-α/β, and modulates NF-κB signaling in macrophages. In the present study, we examined physiological roles of the interaction between STAP-2 and c-Fms in Raw 264.7 macrophage cells. Our immunoprecipitation has revealed that c-Fms directly interacts with the PH domain of STAP-2 independently on M-CSF-stimulation. Ectopic expression of STAP-2 markedly suppressed M-CSF-induced tyrosine phosphorylation of c-Fms as well as activation of Akt and extracellular signal regulated kinase. In addition, Raw 264.7 cells over-expressing STAP-2 showed impaired migration in response to M-CSF and wound-healing process. Taken together, our findings demonstrate that STAP-2 directly binds to c-Fms and interferes with the PI3K signaling, which leads to macrophage motility, in Raw 264.7 cells. |
| Relation: | http://www.sciencedirect.com/science/journal/0006291X |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/22099 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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