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Nuclear retention of STAT3 through the coiled-coil domain regulates its activity.
| Title: | Nuclear retention of STAT3 through the coiled-coil domain regulates its activity. |
| Authors: | Sato, Noriko Browse this author | | Tsuruma, Rieko Browse this author | | Imoto, Seiyu Browse this author | | Sekine, Yuichi Browse this author | | Muromoto, Ryuta Browse this author | | Sugiyama, Kenji Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | IL-6 | | LIF | | STAT3 | | Nuclear translocation | | Phosphorylation | | Transcription |
| Issue Date: | 21-Oct-2005 |
| Publisher: | Elsevier Inc. |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 336 |
| Issue: | 2 |
| Start Page: | 617 |
| End Page: | 624 |
| Publisher DOI: | 10.1016/j.bbrc.2005.08.145 |
| PMID: | 16140268 |
| Abstract: | Signal transducer and activator of transcription 3 (STAT3), which mediates biological actions in many physiological processes, is activated by cytokines and growth factors via specific tyrosine phosphorylation, dimerization, and nuclear translocation. However, the mechanism involved in its nuclear translocation remains unclear. A previous study demonstrated that STAT3 with Arg-214/215 mutations in the coiled-coil domain (R214A/R215A; STAT3 RA) failed to undergo nuclear translocation. Here, we re-examined the nature of the STAT3 RA mutant and found that it showed higher and more extensive tyrosine-phosphorylation as well as much higher STAT3 transcriptional activity in response to stimuli. Furthermore, STAT3 RA showed nuclear translocation and faster nuclear export than wild-type STAT3 after stimulation. Moreover, nuclear retention of STAT3 RA by a chromosomal region maintenance 1 (CRM1) inhibitor, leptomycin B, decreased the enhanced STAT3 activation by stimuli. These data demonstrate that Arg-214/215 are involved in CRM1-mediated STAT3 nuclear export and the regulation of STAT3 activity. |
| Relation: | http://www.sciencedirect.com/science/journal/0006291X |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/22106 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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