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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Information Science and Technology / Faculty of Information Science and Technology >
Peer-reviewed Journal Articles, etc >
Changes in the spontaneous calcium oscillations for the development of the preconditioning-induced ischemic tolerance in neuron/astrocyte co-culture.
| Title: | Changes in the spontaneous calcium oscillations for the development of the preconditioning-induced ischemic tolerance in neuron/astrocyte co-culture. |
| Authors: | Tanaka, Motoki Browse this author | | Kawahara, Koichi Browse this author | | Kosugi, Tatsuro Browse this author | | Yamada, Takeshi Browse this author | | Mioka, Tetsuo Browse this author |
| Keywords: | Spontaneous Ca2+ oscillations | | Ischemic tolerance | | GLT-1 | | PACAP | | Oxygen and glucose deprivation | | Neuron/astrocyte co-cultures |
| Issue Date: | Jun-2007 |
| Publisher: | Springer Netherlands |
| Journal Title: | Neurochemical Research |
| Volume: | 32 |
| Issue: | 6 |
| Start Page: | 988 |
| End Page: | 1001 |
| Publisher DOI: | 10.1007/s11064-006-9259-8 |
| PMID: | 17401678 |
| Abstract: | Spontaneous Ca2+ oscillations are believed to contribute to the regulation of gene expression. Here we investigated whether and how the dynamics of Ca2+ oscillations changed after sublethal preconditioning (PC) for PC-induced ischemic tolerance in neuron/astrocyte co-cultures. The frequency of spontaneous Ca2+ oscillations significantly decreased between 4 and 8 h after the end of PC in both neurons and astrocytes. Treatment with 2-APB, an inhibitor of IP3 receptors, decreased the oscillatory frequency, induced ischemic tolerance and a down-regulation of glutamate transporter GLT-1 contributing to the increase in the extracellular glutamate during ischemia. The expression of GLT-1 is known to be up-regulated by PACAP. Treatment with PACAP38 increased the oscillatory frequency, and antagonized both the PC-induced down-regulation of GLT-1 and ischemic tolerance. These results suggested that the PC suppressed the spontaneous Ca2+ oscillations regulating the gene expressions of various proteins, especially of astrocytic GLT-1, for the development of the PC-induced ischemic tolerance. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/25393 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 河原 剛一
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