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Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA.
| Title: | Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA. |
| Authors: | Inoue, Naonori Browse this author | | Minakawa, Noriaki Browse this author | | Matsuda, Akira Browse this author →KAKEN DB |
| Issue Date: | 2006 |
| Publisher: | Oxford University Press |
| Journal Title: | Nucleic Acids Research |
| Volume: | 34 |
| Issue: | 12 |
| Start Page: | 3476 |
| End Page: | 3483 |
| Publisher DOI: | 10.1093/nar/gkl491 |
| PMID: | 16855286 |
| Abstract: | The synthesis and properties of fully modified 4'-thioDNAs, oligonucleotides consisting of 2'-deoxy-4'-thionucleosides, were examined. In addition to the known literature properties (preferable hybridization with RNA and resistance to endonuclease hydrolysis), we also observed higher resistance of 4'-thioDNA to 3'-exonuclease cleavage. Furthermore, we found that fully modified 4'-thioDNAs behaved like RNA molecules in their hybridization properties and structural aspect, at least in the case of the 4'-thioDNA duplex. This observation was confirmed by experiments using groove binders, in which a 4'-thioDNA duplex interacts with an RNA major groove binder, lividomycin A, but not with DNA groove binders, to give an increase in its thermal stability. Since a 4'-thioDNA duplex competitively inhibited the hydrolysis of an RNA duplex by RNase V1, it was not only the physical properties but also this biological data suggested that a 4'-thioDNA duplex has an RNA-like structure. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/26387 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 南川 典昭
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