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Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA.

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タイトル: Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA.
著者: Inoue, Naonori 著作を一覧する
Minakawa, Noriaki 著作を一覧する
Matsuda, Akira 著作を一覧する
発行日: 2006年
出版者: Oxford University Press
誌名: Nucleic Acids Research
巻: 34
号: 12
開始ページ: 3476
終了ページ: 3483
出版社 DOI: 10.1093/nar/gkl491
抄録: The synthesis and properties of fully modified 4'-thioDNAs, oligonucleotides consisting of 2'-deoxy-4'-thionucleosides, were examined. In addition to the known literature properties (preferable hybridization with RNA and resistance to endonuclease hydrolysis), we also observed higher resistance of 4'-thioDNA to 3'-exonuclease cleavage. Furthermore, we found that fully modified 4'-thioDNAs behaved like RNA molecules in their hybridization properties and structural aspect, at least in the case of the 4'-thioDNA duplex. This observation was confirmed by experiments using groove binders, in which a 4'-thioDNA duplex interacts with an RNA major groove binder, lividomycin A, but not with DNA groove binders, to give an increase in its thermal stability. Since a 4'-thioDNA duplex competitively inhibited the hydrolysis of an RNA duplex by RNase V1, it was not only the physical properties but also this biological data suggested that a 4'-thioDNA duplex has an RNA-like structure.
資料タイプ: article
URI: http://hdl.handle.net/2115/26387
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 南川 典昭

 

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