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Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA.

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Title: Synthesis and properties of 4'-ThioDNA: unexpected RNA-like behavior of 4'-ThioDNA.
Authors: Inoue, Naonori Browse this author
Minakawa, Noriaki Browse this author
Matsuda, Akira Browse this author →KAKEN DB
Issue Date: 2006
Publisher: Oxford University Press
Journal Title: Nucleic Acids Research
Volume: 34
Issue: 12
Start Page: 3476
End Page: 3483
Publisher DOI: 10.1093/nar/gkl491
PMID: 16855286
Abstract: The synthesis and properties of fully modified 4'-thioDNAs, oligonucleotides consisting of 2'-deoxy-4'-thionucleosides, were examined. In addition to the known literature properties (preferable hybridization with RNA and resistance to endonuclease hydrolysis), we also observed higher resistance of 4'-thioDNA to 3'-exonuclease cleavage. Furthermore, we found that fully modified 4'-thioDNAs behaved like RNA molecules in their hybridization properties and structural aspect, at least in the case of the 4'-thioDNA duplex. This observation was confirmed by experiments using groove binders, in which a 4'-thioDNA duplex interacts with an RNA major groove binder, lividomycin A, but not with DNA groove binders, to give an increase in its thermal stability. Since a 4'-thioDNA duplex competitively inhibited the hydrolysis of an RNA duplex by RNase V1, it was not only the physical properties but also this biological data suggested that a 4'-thioDNA duplex has an RNA-like structure.
Type: article
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 南川 典昭

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