Fetal calf serum-free culture of Chinese hamster ovary cells employing fish serum

Fujiwara, M.; Tsukada, R.; Tsujinaga, Y.; Takagi, M.

doi:10.1007/s00253-007-0897-9


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Fetal calf serum-free culture of Chinese hamster ovary cells employing fish serum

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/28048

Title:	Fetal calf serum-free culture of Chinese hamster ovary cells employing fish serum
Authors:	Fujiwara, M. Browse this author →KAKEN DB
	Tsukada, R. Browse this author
	Tsujinaga, Y. Browse this author
	Takagi, M. Browse this author →KAKEN DB
Keywords:	Fish serum
	Fetal calf serum
	Chinese hamster ovary cells
Issue Date:	Jul-2007
Publisher:	Springer
Journal Title:	Applied Microbiology and Biotechnology
Volume:	75
Issue:	5
Start Page:	983
End Page:	987
Publisher DOI:	10.1007/s00253-007-0897-9
PMID:	17334756
Abstract:	The effects of fish serum on cell growth and human granulocyte-macrophage colony-stimulating factor (hGM-CSF) production in an adhesion culture of Chinese hamster ovary (CHO) cells DR1000L4N were investigated and compared with those of fetal calf serum (FCS). Although fish serum did not stimulate the initial adhesion of CHO cells to culture dishes, it prompted cell growth after cell adhesion with FCS for 24 h. The cell density in the fish serum medium reached 75% that in the FCS medium. Fish serum promoted cell adhesion to and cell growth on collagen-coated dishes. The cell-specific production rate of hGM-CSF in the fish serum medium on collagen-coated dishes was almost the same as that in the FCS medium.
Rights:	The original publication is available at www.springerlink.com
Type:	article (author version)
URI:	http://hdl.handle.net/2115/28048
Appears in Collections:	工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高木睦

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