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Daxx enhances Fas-mediated apoptosis in a murine pro-B cell line, BAF3.
| Title: | Daxx enhances Fas-mediated apoptosis in a murine pro-B cell line, BAF3. |
| Authors: | Muromoto, Ryuta Browse this author | | Yamamoto, Tetsuya Browse this author | | Yumioka, Taro Browse this author | | Sekine, Yuichi Browse this author | | Sugiyama, Kenji Browse this author | | Shimoda, Kazuya Browse this author | | Oritani, Kenji Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | Daxx | | Fas | | Apoptosis | | B lymphocyte | | Jun N-terminal kinase | | Interferon |
| Issue Date: | 10-Apr-2003 |
| Publisher: | Elsevier |
| Journal Title: | FEBS Letters |
| Volume: | 540 |
| Issue: | 1-3 |
| Start Page: | 223 |
| End Page: | 228 |
| Publisher DOI: | 10.1016/S0014-5793(03)00269-2 |
| PMID: | 12681512 |
| Abstract: | Daxx has been shown to play an essential in type I interferon (IFN-α/β)-mediated suppression of B cell development and apoptosis. Recently, we demonstrated that Tyk2 is directly involved in IFN signaling for the induction and nuclear translocation of Daxx, which may result in growth arrest and/or apoptosis of B lymphocyte progenitors. To clarify the mechanism of Daxx-mediated apoptosis signaling in B lymphocyte progenitors, here we introduced an efficient suicide switch in a murine pro-B cell line, BAF3, by expressing FK506-binding protein-fused Fas intracellular domain (FKBP-Fas) and Daxx. It allows us to monitor Fas/Daxx-mediated signal by induction of Fas dimerization with the dimerizer drug AP20187. AP20187-mediated Fas dimerization induced not only apoptosis but also Jun N-terminal kinase (JNK) activation. However, AP20187 had no effect on cells expressing either Fas or Daxx only. Furthermore, expression of a JNK inhibitor, the JNK-binding domain of JIP-1, resulted in resistance to AP20187-mediated apoptosis in cells expressing FKBP-Fas and Daxx. These results imply that our novel suicide switch system may provide a powerful tool to delineate or identify the signaling molecules for Daxx-mediated apoptotic machinery in B lymphocyte progenitors through JNK activation. |
| Relation: | http://www.sciencedirect.com/science/journal/00145793 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/28115 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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