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Physical and functional interactions between Daxx and TSG101.

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タイトル: Physical and functional interactions between Daxx and TSG101.
著者: Muromoto, Ryuta 著作を一覧する
Sugiyama, Kenji 著作を一覧する
Yamamoto, Tetsuya 著作を一覧する
Oritani, Kenji 著作を一覧する
Shimoda, Kazuya 著作を一覧する
Matsuda, Tadashi 著作を一覧する
キーワード: Daxx
発行日: 2004年 4月 9日
出版者: Elsevier
誌名: Biochemical and Biophysical Research Communications
巻: 316
号: 3
開始ページ: 827
終了ページ: 833
出版社 DOI: 10.1016/j.bbrc.2004.02.126
抄録: Daxx has been reported to mediate the Fas/JNK-dependent signals in the cytoplasm. However, several evidences have suggested that Daxx is located mainly in the nucleus and functions as a transcriptional regulator. Recently, we identified DMAP1, a TSG101-interacting protein as a Daxx binding partner by yeast two-hybrid screening. TSG101 has been shown to act as transcriptional co-repressor of nuclear hormone receptors. Here we examined whether TSG101also interacts with Daxx directly. The association of Daxx and TSG101 was confirmed using co-expressed tagged proteins. The interaction regions in both proteins were also mapped, and the cellular localization of the interaction was examined. TSG101 formed a complex with Daxx through its coiled-coil domain and co-localized in the nucleus. Furthermore, TSG101 enhanced Daxx-mediated repression of glucocorticoid receptor transcriptional activity. These results provide the novel molecular interactions between Daxx and TSG101, which establish an efficient repressive transcription complex in the nucleus.
Relation (URI):
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 松田 正


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