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Physical and functional interactions between Daxx and TSG101.

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Title: Physical and functional interactions between Daxx and TSG101.
Authors: Muromoto, Ryuta Browse this author
Sugiyama, Kenji Browse this author
Yamamoto, Tetsuya Browse this author
Oritani, Kenji Browse this author
Shimoda, Kazuya Browse this author
Matsuda, Tadashi Browse this author →KAKEN DB
Keywords: Daxx
Issue Date: 9-Apr-2004
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 316
Issue: 3
Start Page: 827
End Page: 833
Publisher DOI: 10.1016/j.bbrc.2004.02.126
PMID: 15033475
Abstract: Daxx has been reported to mediate the Fas/JNK-dependent signals in the cytoplasm. However, several evidences have suggested that Daxx is located mainly in the nucleus and functions as a transcriptional regulator. Recently, we identified DMAP1, a TSG101-interacting protein as a Daxx binding partner by yeast two-hybrid screening. TSG101 has been shown to act as transcriptional co-repressor of nuclear hormone receptors. Here we examined whether TSG101also interacts with Daxx directly. The association of Daxx and TSG101 was confirmed using co-expressed tagged proteins. The interaction regions in both proteins were also mapped, and the cellular localization of the interaction was examined. TSG101 formed a complex with Daxx through its coiled-coil domain and co-localized in the nucleus. Furthermore, TSG101 enhanced Daxx-mediated repression of glucocorticoid receptor transcriptional activity. These results provide the novel molecular interactions between Daxx and TSG101, which establish an efficient repressive transcription complex in the nucleus.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松田 正

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