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Physical and functional interactions between Daxx and TSG101.
| Title: | Physical and functional interactions between Daxx and TSG101. |
| Authors: | Muromoto, Ryuta Browse this author | | Sugiyama, Kenji Browse this author | | Yamamoto, Tetsuya Browse this author | | Oritani, Kenji Browse this author | | Shimoda, Kazuya Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | Daxx | | TSG101 | | Transcription | | Repression |
| Issue Date: | 9-Apr-2004 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 316 |
| Issue: | 3 |
| Start Page: | 827 |
| End Page: | 833 |
| Publisher DOI: | 10.1016/j.bbrc.2004.02.126 |
| PMID: | 15033475 |
| Abstract: | Daxx has been reported to mediate the Fas/JNK-dependent signals in the cytoplasm. However, several evidences have suggested that Daxx is located mainly in the nucleus and functions as a transcriptional regulator. Recently, we identified DMAP1, a TSG101-interacting protein as a Daxx binding partner by yeast two-hybrid screening. TSG101 has been shown to act as transcriptional co-repressor of nuclear hormone receptors. Here we examined whether TSG101also interacts with Daxx directly. The association of Daxx and TSG101 was confirmed using co-expressed tagged proteins. The interaction regions in both proteins were also mapped, and the cellular localization of the interaction was examined. TSG101 formed a complex with Daxx through its coiled-coil domain and co-localized in the nucleus. Furthermore, TSG101 enhanced Daxx-mediated repression of glucocorticoid receptor transcriptional activity. These results provide the novel molecular interactions between Daxx and TSG101, which establish an efficient repressive transcription complex in the nucleus. |
| Relation: | http://www.sciencedirect.com/science/journal/0006291X |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/28120 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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