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Determination of the transphosphorylation sites of Jak2 kinase.

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タイトル: Determination of the transphosphorylation sites of Jak2 kinase.
著者: Matsuda, Tadashi 著作を一覧する
Feng, Jian 著作を一覧する
Witthuhn, Bruce A. 著作を一覧する
Sekine, Yuichi 著作を一覧する
Ihle, James N. 著作を一覧する
キーワード: Jak
Transphosphorylation
発行日: 2004年12月10日
出版者: Elsevier
誌名: Biochemical and Biophysical Research Communications
巻: 325
号: 2
開始ページ: 586
終了ページ: 594
出版社 DOI: 10.1016/j.bbrc.2004.10.071
抄録: Janus kinases are the key enzymes involved in the initial transmission of signals in response to type I and II cytokines. Activation of the signal begins with the transphosphorylation of Jak kinases. Substrates that give rise to downstream events are recruited to the receptor complex in part by interactions with phosphorylated tyrosines. The identity of many of the phosphotyrosines responsible for recruitment has been elucidated as being receptor-based tyrosines. The ability of Jaks to recruit substrates through their own phosphotyrosines has been demonstrated for tyrosines in the kinase activation loop. Recent studies demonstrate that other tyrosines have implications in regulatory roles of Jak kinase activity. In this study, baculovirus-produced Jak2 was utilized to demonstrate that transphosphorylation of Jak kinases occurs on multiple residues throughout the protein. We demonstrate that among the tyrosines phosphorylated, those in the kinase domain occur as expected, but many other sites are also phosphorylated. The tyrosines conserved in the Jak family are the object of this study, although many of them are phosphorylated, many are not. This result suggests that conservation of tyrosines is perhaps as important in maintaining structure of the Jak family. Additionally, non-Jak family conserved tyrosines are phosphorylated suggesting that the individual Jaks ability to phosphorylated specific tyrosines may influence signals emitting from activated Jaks.
Relation (URI): http://www.sciencedirect.com/science/journal/0006291X
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/28122
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 松田 正

 

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