Hokkaido University Collection of Scholarly and Academic Papers >
Faculty of Pharmaceutical Sciences >
Peer-reviewed Journal Articles, etc >
Regulation of transforming growth factor-β signaling by protein inhibitor of activated STAT, PIASy through Smad3.
Title: | Regulation of transforming growth factor-β signaling by protein inhibitor of activated STAT, PIASy through Smad3. |
Other Titles: | Regulation of TGF-β Signaling by Protein Inhibitor Activated of STAT, PIASy through Smad3 |
Authors: | Imoto, Seiyu Browse this author | Sugiyama, Kenji Browse this author | Muromoto, Ryuta Browse this author | Sato, Noriko Browse this author | Yamamoto, Tetsuya Browse this author | Matsuda, Tadashi Browse this author |
Issue Date: | 5-Sep-2003 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Journal Title: | Journal of Biological Chemistry |
Volume: | 278 |
Issue: | 36 |
Start Page: | 34253 |
End Page: | 34258 |
Publisher DOI: | 10.1074/jbc.M304961200 |
PMID: | 12815042 |
Abstract: | Smads proteins play a key role in the intracellular signaling of the transforming growth factor (TGF)-β family of growth factors, which exhibits a diverse set of cellular responses, including cell proliferation and differentiation. In particular, Smad7 acts as an antagonist of TGF-β signaling, which could determine the intensity or duration of its signaling cascade. In this study we identified a protein inhibitor of activated STAT (signal transducers and activators of transcription), PIASy, as a novel interaction partner of Smad7 by yeast two-hybrid screening using the MH2 domain of Smad7 as bait. The association of Smad7 and PIASy was confirmed using co-expressed tagged proteins in 293T cells. Moreover, we found that other Smads including Smad3 also associated with PIASy through its MH2 domain, and PIASy suppressed TGF-β-mediated activation of Smad3. PIASy also stimulated the sumoylation of Smad3 in vivo. Furthermore, endogenous PIASy expression was induced by TGF-β in Hep3B cells. These findings provide the first evidence that a PIAS family protein, PIASy, associates with Smads and involves the regulation of TGF-β signaling using the negative feedback loop. |
Rights: | Copyright © 2003 by the American Society for Biochemistry and Molecular Biology |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/28123 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 松田 正
|