RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells

Hoshika, Shuichi; Minakawa, Noriaki; Kamiya, Hiroyuki; Harashima, Hideyoshi; Matsuda, Akira

doi:10.1016/j.febslet.2005.04.073


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RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells

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Title:	RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells
Other Titles:	RNA interference (RNAi) induced by siRNAs modified with 4'-thioribonucleosides incultured mammalian cells
Authors:	Hoshika, Shuichi Browse this author
	Minakawa, Noriaki Browse this author
	Kamiya, Hiroyuki Browse this author
	Harashima, Hideyoshi Browse this author →KAKEN DB
	Matsuda, Akira Browse this author →KAKEN DB
Keywords:	RNA interference (RNAi)
	Short interfering RNA (siRNA)
	Chemical modification
	4'-thioribonucleoside
	4'-thioRNA
Issue Date:	6-Jun-2005
Publisher:	Elsevier B.V.
Journal Title:	FEBS Letters
Volume:	579
Issue:	14
Start Page:	3115
End Page:	3118
Publisher DOI:	10.1016/j.febslet.2005.04.073
PMID:	15919084
Abstract:	Short interfering RNAs (siRNAs) variously modified with 4'-thioribonucleosides against the Photinus luciferase gene were tested for their induction of the RNA interference (RNAi) activity in cultured NIH/3T3 cells. Results indicated that modifications at the sense-strand were well tolerated for RNAi activity except for full modification with 4'-thioribonucleosides. However, the activity of siRNAs modified at the antisense-strand was dependent on the position and the number of modifications with 4'-thioribonucleosides. Since modifications of siRNAs with 4'-thioribonucleosides were well tolerated in RNAi activity compared with that of 2'-O-methyl nucleosides, 4'-thioribonucleosides might be potentially useful in the development of novel and effective chemically modified siRNAs.
Relation:	http://www.sciencedirect.com/science/journal/00145793
Type:	article (author version)
URI:	http://hdl.handle.net/2115/30353
Appears in Collections:	薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 南川典昭

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