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RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells

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Title: RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells
Other Titles: RNA interference (RNAi) induced by siRNAs modified with 4'-thioribonucleosides incultured mammalian cells
Authors: Hoshika, Shuichi Browse this author
Minakawa, Noriaki Browse this author
Kamiya, Hiroyuki Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Matsuda, Akira Browse this author →KAKEN DB
Keywords: RNA interference (RNAi)
Short interfering RNA (siRNA)
Chemical modification
Issue Date: 6-Jun-2005
Publisher: Elsevier B.V.
Journal Title: FEBS Letters
Volume: 579
Issue: 14
Start Page: 3115
End Page: 3118
Publisher DOI: 10.1016/j.febslet.2005.04.073
PMID: 15919084
Abstract: Short interfering RNAs (siRNAs) variously modified with 4'-thioribonucleosides against the Photinus luciferase gene were tested for their induction of the RNA interference (RNAi) activity in cultured NIH/3T3 cells. Results indicated that modifications at the sense-strand were well tolerated for RNAi activity except for full modification with 4'-thioribonucleosides. However, the activity of siRNAs modified at the antisense-strand was dependent on the position and the number of modifications with 4'-thioribonucleosides. Since modifications of siRNAs with 4'-thioribonucleosides were well tolerated in RNAi activity compared with that of 2'-O-methyl nucleosides, 4'-thioribonucleosides might be potentially useful in the development of novel and effective chemically modified siRNAs.
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 南川 典昭

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