HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >

Deposition transfection technology using a DNA complex with a thermoresponsive cationic star polymer

Files in This Item:
JOCR123-3.pdf389.32 kBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Deposition transfection technology using a DNA complex with a thermoresponsive cationic star polymer
Other Titles: Deposition transfection technology using a DNA complex with a thermoresponsive cationic Branched polymer
Authors: Zhou, Yue-Min Browse this author
Ishikawa, Ayaka Browse this author
Okahashi, Ryohei Browse this author
Uchida, Kingo Browse this author
Nemoto, Yasushi Browse this author
Nakayama, Mitsuko Browse this author
Nakayama, Yasuhide Browse this author
Keywords: DNA delivery
reverse transfection
deposition transfection
thermoresponsive polymer
cationic polymer
Issue Date: 20-Jan-2007
Journal Title: Journal of Controlled Release
Volume: 123
Issue: 3
Start Page: 239
End Page: 246
Publisher DOI: 10.1016/j.jconrel.2007.08.026
Abstract: A novel non-viral gene transfection method in which DNA complexes were kept in contact with a deposition surface (deposition transfection) was developed. We designed a novel aqueous thermoresponsive adsorbent material for DNA deposition, which was a star-shaped copolymer with 4-branched chains. Each chain is comprised of a cationic poly(N,N-dimethylaminopropyl acrylamide) (PDMAPAAm) block (Mn: ca. 3000 g center dot mol(-1)), which formed an inner domain for DNA binding and a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) block (Mn: ca. 6000 g center dot mol(-1)), which formed an outer domain for surface adsorption. Complex formation between the copolymer and the luciferase-encoding plasmid DNA occurred immediately upon simple mixing in an aqueous medium; polyplexes approximately 100 nm in size were formed. Because the lower critical solution temperature of the polyplexes was approximately 35 degrees C, they could deposit on the substrate by precipitation from an aqueous solution upon warming, which was confirmed by quartz crystal microbalance (QCM) method and water contact angle measurement. When COS-I cells were cultured on the polyplex-deposited substrate in a culture medium, the luciferase activity observed was higher than that observed on a DNA-coated substrate with or without the cationic polymer before and after complete adhesion and by conventional solution transfection using the polyplexes. The activity was enhanced with an increase in the charge ratio (surfactant/pDNA) with permissible cellular cytotoxicity. (c) 2007 Elsevier B.V. All rights reserved.
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中山 泰秀

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University