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UvrA and UvrB enhance mutations induced by oxidized deoxyribonucleotides

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タイトル: UvrA and UvrB enhance mutations induced by oxidized deoxyribonucleotides
著者: Hori, Mika 著作を一覧する
Ishiguro, Chieko 著作を一覧する
Suzuki, Tetsuya 著作を一覧する
Nakagawa, Noriko 著作を一覧する
Nunoshiba, Tatsuo 著作を一覧する
Kuramitsu, Seiki 著作を一覧する
Yamarnoto, Kazuo 著作を一覧する
Kasai, Hiroshi 著作を一覧する
Harashima, Hideyoshi 著作を一覧する
Kamiya, Hiroyuki 著作を一覧する
キーワード: UvrA
発行日: 2007年12月 1日
出版者: Elsevier
誌名: DNA Repair
巻: 6
号: 12
開始ページ: 1786
終了ページ: 1793
出版社 DOI: 10.1016/j.dnarep.2007.06.013
抄録: Oxidatively damaged DNA precursors (deoxyribonucleotides) are formed by reactive oxygen species. After the damaged DNA precursors are incorporated into DNA, they might be removed by DNA repair enzymes. in this study, to examine whether a nucleotide excision repair enzyme, Escherichia coli UvrABC, could suppress the mutations induced by oxidized deoxyribonucleotides in vivo, oxidized DNA precursors, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate, were introduced into uvrA, uvrB, and uvrC E. coli strains, and mutations in the chromosomal rpoB gene were analyzed. Unexpectedly, these oxidized DNA precursors induced mutations only slightly in the uvrA and uvrB strains. In contrast, effect of the uvrC-deficiency was not observed. Next, mutT, mutT/uvrA, and mutT/uvrB E. coli strains were treated with H_{2}O_{2}, and the rpoB mutant frequencies were calculated. The frequency of the H_{2}O_{2}-induced mutations was increased in all of the strains tested; however, the increase was three- to four-fold lower in the mutT/uvrA and mutT/uvrB strains than in the mutT strain. Thus, UvrA and UvrB are involved in the enhancement, but not in the suppression, of the mutations induced by these oxidized deoxyribonucleotides. These results suggest a novel role for UvrA and UvrB in the processing of oxidative damage.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 紙谷 浩之


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