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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Genomic Structure and Expression of the Sea Urchin Soluble Guanylyl Cyclase β Subunit Gene
| Title: | Genomic Structure and Expression of the Sea Urchin Soluble Guanylyl Cyclase β Subunit Gene |
| Authors: | Tanabe, Yasunori Browse this author | | Suzuki, Norio Browse this author |
| Keywords: | soluble guanylyl cyclase | | sea urchin | | genome | | cDNA | | Hemicentrotus pulcherrimus |
| Issue Date: | Aug-2001 |
| Publisher: | 日本動物学会 |
| Journal Title: | Zoological Science |
| Volume: | 18 |
| Issue: | 6 |
| Start Page: | 811 |
| End Page: | 817 |
| Abstract: | We obtained the full-length cDNA and genomic DNA clones of HpGCS-β1 encoding theβsubunit of soluble guanylyl cyclase (soluble GC), which is expressed in the testis and ovary of the sea urchin Hemicentrotus pulcherrimus. Reverse transcription-polymerase chain reaction analysis demonstrated that the HpGCS-β1 transcript was detected in unfertilized eggs as well as in the testis and ovary. The open reading frame predicted a protein of 604 amino acids with a putative cyclase catalytic domain conserved in membrane GCs and adenylyl cyclases. 105His, 78Cys, and 122Cys, important amino acids for heme-binding in theβ1 subunit of soluble GCs in vertebrates, were conserved in the corresponding positions of HpGCS-β1. The HpGCS-β1 gene consisted of 8 exons and had a span of 26 kbp. A comparison of genomic structure of the HpGCS-β1 gene with that of the soluble GCβ1 subunit gene (OlGCS-β1) of the medaka fish indicated that exon 4 in the HpGCS-β1 gene corresponds to exons 4 to 9 in the OlGCS-β1 gene. |
| Rights: | (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである |
| Relation: | http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/32949 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 範男
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