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Genomic Structure and Expression of the Sea Urchin Soluble Guanylyl Cyclase β Subunit Gene

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Title: Genomic Structure and Expression of the Sea Urchin Soluble Guanylyl Cyclase β Subunit Gene
Authors: Tanabe, Yasunori Browse this author
Suzuki, Norio Browse this author
Keywords: soluble guanylyl cyclase
sea urchin
genome
cDNA
Hemicentrotus pulcherrimus
Issue Date: Aug-2001
Publisher: 日本動物学会
Journal Title: Zoological Science
Volume: 18
Issue: 6
Start Page: 811
End Page: 817
Abstract: We obtained the full-length cDNA and genomic DNA clones of HpGCS-β1 encoding theβsubunit of soluble guanylyl cyclase (soluble GC), which is expressed in the testis and ovary of the sea urchin Hemicentrotus pulcherrimus. Reverse transcription-polymerase chain reaction analysis demonstrated that the HpGCS-β1 transcript was detected in unfertilized eggs as well as in the testis and ovary. The open reading frame predicted a protein of 604 amino acids with a putative cyclase catalytic domain conserved in membrane GCs and adenylyl cyclases. 105His, 78Cys, and 122Cys, important amino acids for heme-binding in theβ1 subunit of soluble GCs in vertebrates, were conserved in the corresponding positions of HpGCS-β1. The HpGCS-β1 gene consisted of 8 exons and had a span of 26 kbp. A comparison of genomic structure of the HpGCS-β1 gene with that of the soluble GCβ1 subunit gene (OlGCS-β1) of the medaka fish indicated that exon 4 in the HpGCS-β1 gene corresponds to exons 4 to 9 in the OlGCS-β1 gene.
Rights: (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである
Relation: http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003
Type: article
URI: http://hdl.handle.net/2115/32949
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 範男

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