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Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes
Title: | Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes |
Authors: | Yao, Yuko Browse this author | Yamamoto, Takehiro Browse this author | Tsutsumi, Makiko Browse this author | Matsuda, Masaru Browse this author | Hori, Hiroshi Browse this author | Naruse, Kiyoshi Browse this author | Mitani, Hiroshi Browse this author | Shima, Akihiro Browse this author | Asakawa, Shuichi Browse this author | Shimizu, Nobuyoshi Browse this author | Suzuki, Norio Browse this author |
Keywords: | soluble guanylyl cyclase | cGMP | medaka fish | gene expression | exon/intron organization |
Issue Date: | Oct-2003 |
Publisher: | 日本動物学会 |
Journal Title: | Zoological Science |
Volume: | 20 |
Issue: | 10 |
Start Page: | 1293 |
End Page: | 1304 |
Abstract: | A cDNA clone encoding the soluble guanylyl cyclase α2 subunit was isolated from medaka fish (Oryzias latipes) and designated as OIGCS-α2. The OIGCS-α2 cDNA was 3192 bp in length and the open reading frame (ORF) encodes a protein of 805 amino acids. The deduced amino acid sequence has high similarity to that of the mammalianα2 subunit gene except for the N-terminal regulatory domain. The C-terminal 5 amino acids, "RETSL", which have been reported to interact with the post synaptic density protein (PSD)-95 were conserved. An RNase protection assay with adult fish organs showed that O/GCS-α2 was expressed mainly in the brain and testis. The complete nucleotide sequence (about 41 kbp) of the OIGCS-α2 genomic DNA clone isolated from a medaka fish BAC library indicated that the OIGCS-α2 gene consisted of 9 exons and 8 introns. The 5'-flanking region and larger introns, such as introns 1, 4, and 7, contained the several fragments conserved in the nucleotide sequences of Rex6 (non-long terminal repeat retrotransposon), MHC class I genomic region, and OIGC1, the medaka fish homolog of the mammalian guanylyl cyclase B gene. Linkage analysis on the medaka fish chromosome demonstrated that the OIGCS-α2 gene was mapped to LG13; this mapping position was different from those for the OIGCS-α1 and OIGCS-β1 genes (LG1). |
Rights: | (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである |
Relation: | http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003 |
Type: | article |
URI: | http://hdl.handle.net/2115/32965 |
Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 範男
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