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Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes

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Title: Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes
Authors: Yao, Yuko Browse this author
Yamamoto, Takehiro Browse this author
Tsutsumi, Makiko Browse this author
Matsuda, Masaru Browse this author
Hori, Hiroshi Browse this author
Naruse, Kiyoshi Browse this author
Mitani, Hiroshi Browse this author
Shima, Akihiro Browse this author
Asakawa, Shuichi Browse this author
Shimizu, Nobuyoshi Browse this author
Suzuki, Norio Browse this author
Keywords: soluble guanylyl cyclase
medaka fish
gene expression
exon/intron organization
Issue Date: Oct-2003
Publisher: 日本動物学会
Journal Title: Zoological Science
Volume: 20
Issue: 10
Start Page: 1293
End Page: 1304
Abstract: A cDNA clone encoding the soluble guanylyl cyclase α2 subunit was isolated from medaka fish (Oryzias latipes) and designated as OIGCS-α2. The OIGCS-α2 cDNA was 3192 bp in length and the open reading frame (ORF) encodes a protein of 805 amino acids. The deduced amino acid sequence has high similarity to that of the mammalianα2 subunit gene except for the N-terminal regulatory domain. The C-terminal 5 amino acids, "RETSL", which have been reported to interact with the post synaptic density protein (PSD)-95 were conserved. An RNase protection assay with adult fish organs showed that O/GCS-α2 was expressed mainly in the brain and testis. The complete nucleotide sequence (about 41 kbp) of the OIGCS-α2 genomic DNA clone isolated from a medaka fish BAC library indicated that the OIGCS-α2 gene consisted of 9 exons and 8 introns. The 5'-flanking region and larger introns, such as introns 1, 4, and 7, contained the several fragments conserved in the nucleotide sequences of Rex6 (non-long terminal repeat retrotransposon), MHC class I genomic region, and OIGC1, the medaka fish homolog of the mammalian guanylyl cyclase B gene. Linkage analysis on the medaka fish chromosome demonstrated that the OIGCS-α2 gene was mapped to LG13; this mapping position was different from those for the OIGCS-α1 and OIGCS-β1 genes (LG1).
Rights: (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 範男

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