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Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae

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Title: Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae
Authors: Kobayashi, Yasuhisa Browse this author
Kobayashi, Tohru Browse this author
Nakamura, Masaru Browse this author
Sunobe, Tomoki Browse this author
Morrey, Craig E. Browse this author
Suzuki, Norio Browse this author
Nagahama, Yoshitaka Browse this author
Keywords: aromatase genes
gonadal development and maturation
sex-changing fish
gobiid fish
Issue Date: Apr-2004
Publisher: 日本動物学会
Journal Title: Zoological Science
Volume: 21
Issue: 4
Start Page: 417
End Page: 425
Abstract: To investigate the role of estrogen in the serial-sex changing fish Trimma okinawae, we isolated complementary DNAs encoding two distinct cytochrome P450 aromatase isoforms from adult ovary and brain (termed P450aromA and P450aromB, respectively). Sequence and phylogenic analyses showed that the goby P450arom forms belong to two separate CYP19 subfamilies. Transient expression of these cDNAs in HEK293 cells caused conversion of exogenous testosterone to estradiol-17β. RT-PCR showed that P450aromA was expressed in the brain, spleen, testis and ovary. P450aromB was expressed in the brain, liver, testis and ovary. In situ hybridization studies showed that P450aromA mRNA, but not P450aromB mRNA, was present in both ovary and testis. Positive signals were restricted to granulosa cells of vitellogenic follicles and interstitial cells of mature testis. Ovarian expression of both P450arom genes during the spawning cycle was examined by quantitative real-time RT-PCR. P450aromA transcripts increased during vitellogenesis and decreased prior to spawning. In contrast, P450aromB transcripts were barely detectable and did not correlate with ovarian development. These findings suggest that P450aromA, but not P450aromB, is involved in regulating ovarian vitellogenesis in goby.
Rights: (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである
Relation: http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003
Type: article
URI: http://hdl.handle.net/2115/32967
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 範男

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