HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Field Science Center for Northern Biosphere >
Peer-reviewed Journal Articles, etc >

Seasonal Variation in the Expression of Five Subtypes of Gonadotropin-releasing Hormone Receptor Genes in the Brain of Masu Salmon from Immaturity to Spawning

Files in This Item:
i0289-0003-22-12-1331.pdf391.55 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/32970

Title: Seasonal Variation in the Expression of Five Subtypes of Gonadotropin-releasing Hormone Receptor Genes in the Brain of Masu Salmon from Immaturity to Spawning
Authors: Jodo, Aya Browse this author
Kitahashi, Takashi Browse this author
Taniyama, Shinya Browse this author
Bhandari, Ramji Kumar Browse this author
Ueda, Hiroshi Browse this author →KAKEN DB
Urano, Akihisa Browse this author
Ando, Hironori Browse this author
Keywords: GnRH receptor
gene expression
brain
seasonal reproduction
spawning behavior
real-time PCR
Issue Date: Dec-2005
Publisher: 日本動物学会
Journal Title: Zoological Science
Volume: 22
Issue: 12
Start Page: 1331
End Page: 1338
Abstract: Seasonal variation in the expression of five subtypes of gonadotropin-releasing hormone receptor (GnRH-R) genes, designated as msGnRH-R1, -R2, -R3, -R4, and -R5, was examined in the brain of masu salmon (Oncorhynchus masou). In addition, responses of these genes to GnRH were examined in a GnRH analog (GnRHa) implantation experiment. Brain samples were collected one week after the implantation every month from immaturity through spawning. The absolute amount of GnRH-R mRNA in single forebrains was determined by real-time PCR assays. Among the five genes, R4 and R5 were dominantly expressed in both sexes. R1, R4, and R5 mRNAs showed similar changes throughout the experimental period in both sexes. Levels tended to be high in winter and low in the pre-spawning season, followed by elevations in the spawning period. The mRNA levels had weak to moderate negative correlations with the plasma level of estradiol-17β (E2) in females. The effects of GnRHa on msGnRH-R mRNAs were not apparent for all the subtypes. These results indicate that the msGnRH-R1, -R4, and -R5 genes are synchronously expressed during sexual maturation. There was a trend toward decreased levels of their expression prior to the spawning period and then increased levels at spawning, possibly causing GnRH target neurons to sensitize to a GnRH stimulus. Furthermore, E2 may be involved in msGnRH-R gene expression in the brain of female masu salmon during sexual maturation.
Rights: (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである
Relation: http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003
Type: article
URI: http://hdl.handle.net/2115/32970
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 上田 宏

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University