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Characterization of Sea Urchin Sperm Membrane Proteins which Interact with a Major Acrosome Reaction-Inducing Substance, Fucose Sulfate Glycoconjugate

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Title: Characterization of Sea Urchin Sperm Membrane Proteins which Interact with a Major Acrosome Reaction-Inducing Substance, Fucose Sulfate Glycoconjugate
Authors: Satoh, Yuichi Browse this author
Shimizu, Takeshi Browse this author
Harumi, Tatsuo Browse this author
Suzuki, Norio Browse this author
Issue Date: Jun-1996
Publisher: 日本動物学会
Journal Title: Zoological Science
Volume: 13
Issue: 3
Start Page: 377
End Page: 383
Abstract: Intact sea urchin spermatozoa were successfully biotinylated with NHS-LC-Biotin and the biotinylated spermatozoa retained the viability. Analysis of the membrane prepared from the biotinylated spermatozoa of the sea urchin Hemicentrotus pulcherrimus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that several proteins such as wheat-germ agglutinin (WGA)-binding protein (220 kDa), guanylyl cyclase (131 kDa), sperm-activating peptide-I (SAP-I)-crosslinking protein (71 kDa), GPI-anchored protein (63 kDa) and functionally unknown proteins (50 kDa and 30 kDa) were specifically biotinylated. Experiments using spermatozoa of sea urchins, Anthocidaris crassispina and Clypeasfer japonicus showed that several proteins similar to those of H. pulcherrimus spermatozoa were also labeled with NHS-LC-Biotin. Fucose sulfate glycoconjugate (FSG) isolated from the jelly coat of H. pulcherrimus was mixed with solubilized biotinylated sperm membrane proteins of H. pulcherrimus, A. crassispina or C. japonicus and then subjected to gel filtration chromatography on a Sepharose 2B column, indicating that only two biotinylated H. pulcherrimus sperm proteins were coeluted with H. pulcherrimus FSG.
Rights: (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである
Relation: http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003
Type: article
URI: http://hdl.handle.net/2115/32992
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 範男

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