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Dephosphorylation of Autophosphorylated Regulatory Subunit of Sea Urchin Sperm cAMP-Dependent Histone Kinase by an Endogenous Protein Phosphatase
Title: | Dephosphorylation of Autophosphorylated Regulatory Subunit of Sea Urchin Sperm cAMP-Dependent Histone Kinase by an Endogenous Protein Phosphatase |
Authors: | Hoshino, Katsuaki Browse this author | Suzuki, Norio Browse this author |
Issue Date: | Oct-1996 |
Publisher: | 日本動物学会 |
Journal Title: | Zoological Science |
Volume: | 13 |
Issue: | 5 |
Start Page: | 711 |
End Page: | 718 |
Abstract: | Seven protein phosphatases were isolated from extracts of spermatozoa. and sperm tails of the sea urchin Hemicentrotus pulcherrimus by ion exchange and gel filtration chromatographies using [32P]-histone and/or p-nitrophenyl phosphate (pNPP) as substrates and characterized for their enzymatic and molecular nature. Two of them isolated from the particulate fraction correspond to the mammalian type 1 and 2B protein phosphatases. Another two obtained from the soluble fraction were similar to the mammalian protein phosphatases, type 2A and 2C. A protein phosphatase corresponding to the mammalian type 1 enzyme (molecular mass of 43 kDa) dephosphorylated the [32P]-autophosphorylated regulatory subunit of H. pulcherrimus sperm cAMP-dependent histone kinase. |
Rights: | (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである |
Relation: | http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003 |
Type: | article |
URI: | http://hdl.handle.net/2115/32997 |
Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 範男
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