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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Mass Spectrometric Analysis of Phosphoserine Residues Conserved in the Catalytic Domain of Membrane-Bound Guanylyl Cyclase from the Sea Urchin Spermatozoa
| Title: | Mass Spectrometric Analysis of Phosphoserine Residues Conserved in the Catalytic Domain of Membrane-Bound Guanylyl Cyclase from the Sea Urchin Spermatozoa |
| Authors: | Furuya, Hirotaka Browse this author | | Yoshino, Ken-ichi Browse this author | | Shimizu, Takeshi Browse this author | | Mantoku, Tsuyoshi Browse this author | | Takeda, Tae Browse this author | | Nomura, Kohji Browse this author | | Suzuki, Norio Browse this author |
| Issue Date: | Aug-1998 |
| Publisher: | 日本動物学会 |
| Journal Title: | Zoological Science |
| Volume: | 15 |
| Issue: | 4 |
| Start Page: | 507 |
| End Page: | 516 |
| Publisher DOI: | 10.2108/0289-0003(1998)15[507:msaopr]2.0.co;2 |
| Abstract: | We have developed a large-scale purification method of the phosphorylated form (131 kDa) of membrane-bound guanylyl cyclase (mGC) from Hemicentrotus pulcherrimus spermatozoa. The purified mGC contained 26.0 ±1.3 moles of phosphate/mol enzyme (mean ±S.D., n = 6). Phosphorylated peptides were isolated from the trypsin digest of the carboxymethylated Hi. pulcherrimus sperm mGC by affinity chromatography on a Chelating Sepharose Fast Flow column, and the peptides were then subjected to mass spectrometric analysis and determination of phosphoserines, after the conversion of phosphoserines to S-ethylcysteines by amino acid analysis. Based on the observed mass number and the content of phosphoserine, serine residues at positions 561, 565, 652, 722, 740, 755, 894, 897, 914, 918, 927, 930, 951, and 985, in addition to two residues among those at positions 666, 670, and 671, were shown to be phosphorylated. They are all located in the intracellular region (kinase-like and catalytic domains). Notably, serine residues at positions 894, 918, 927, and 930, that are conserved in the sequence of mammalian mGCs and medaka fish-eye-specific mGCs, are phosphorylated in the sea urchin sperm mGC. |
| Rights: | (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである |
| Relation: | http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/33001 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 範男
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