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Improvement of Force Modulation Mode with Scanning Probe Microscopy for Imaging Viscoelasticity of Living Cells
| Title: | Improvement of Force Modulation Mode with Scanning Probe Microscopy for Imaging Viscoelasticity of Living Cells |
| Authors: | Nagayama, Masafumi Browse this author →KAKEN DB | | Haga, Hisashi Browse this author →KAKEN DB | | Tanaka, Yoshio Browse this author | | Hirai, Yoshihiko Browse this author | | Kabuto, Masaaki Browse this author | | Kawabata, Kazushige Browse this author |
| Keywords: | atomic force microscopy | | cell dynamics | | mechanical property | | elasticity | | Young's modulus | | viscosity | | fibroblast |
| Issue Date: | Jul-2002 |
| Publisher: | Japan Society of Applied Physics |
| Journal Title: | Japanese Journal of Applied Physics. Pt. 1, Regular papers, short notes & review papers |
| Volume: | 41 |
| Issue: | 7B |
| Start Page: | 4952 |
| End Page: | 4955 |
| Publisher DOI: | 10.1143/JJAP.41.4952 |
| Abstract: | We improved the force modulation mode with scanning probe microscopy (SPM) in order to make a quantitative evaluation of the viscoelasticity of living cells. Taking account of the viscosity of liquid medium, the vibration frequency of the cantilever was selected to be 500 Hz, and analysis of cantilever vibration was adopted for evaluation of the viscoelasticity of the samples. Consequently, we have succeeded in determining viscoelasticity distribution on living cells. The values of Young's modulus and the coefficient of viscosity vary from 10 to 50 kPa and from 20 to 40 Pa·s on a cell, depending on its internal cellular structure. |
| Rights: | Copyright © 2002 The Japan Society of Applied Physics |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/33072 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永山 昌史
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