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Interaction of the Halobacterial Transducer to a Halorhodopsin Mutant Engineered so as to Bind the Transducer : Cl- Circulation within the Extracellular Channel

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Title: Interaction of the Halobacterial Transducer to a Halorhodopsin Mutant Engineered so as to Bind the Transducer : Cl- Circulation within the Extracellular Channel
Authors: Hasegawa, Chisa Browse this author
Kikukawa, Takashi Browse this author →KAKEN DB
Miyauchi, Seiji Browse this author
Seki, Akiteru Browse this author
Sudo, Yuki Browse this author
Kubo, Megumi Browse this author
Demura, Makoto Browse this author
Kamo, Naoki Browse this author
Issue Date: Mar-2007
Publisher: Blackwell Publishing
Journal Title: Photochemistry and Photobiology
Volume: 83
Issue: 2
Start Page: 293
End Page: 302
Publisher DOI: 10.1562/2006-06-09-RA-916
PMID: 16978043
Abstract: An alkali-halophilic archaeum, Natronomonas pharaonis, contains two rhodopsins that are halorhodopsin (phR), a light-driven inward Cl- pump and phoborhodopsin (ppR), the receptor of negative phototaxis functioning by forming a signaling complexes with a transducer, pHtrII. Previously, we reported that the phR double mutant, P240T/F250YphR, can bind with pHtrII [Y. Sudo et al. (2006) J. Mol. Biol. 357, 1274-1282]. This mutant itself can transport Cl-, while the net transport was stopped upon formation of the complex. The flash photolysis data were analyzed by a scheme in which phR ⇒ P1 → P2 → P3 → P4 → phR. The P3 of the wild-type and the double mutant contained two components, X- and O-intermediates. After the complex formation, however, the P3 of the double mutant lacked the X-intermediate. These observations imply that the X-intermediate (probably the N-intermediate) is the state having Cl- in the cytoplasmic binding site and that the complex undergoes an extracellular Cl- circulation due to the inhibition of formation of the X-intermediate.
Rights: Author Posting. © The Authors 2007 This is the author's version of the work. It is posted here for personal use, not for redistribution. The definitive version was published in Photochemistry and Photobiology, Volume 83, Issue 2, Page 293-302. http://dx.doi.org/10.1562/2006-06-09-RA-916
Type: article (author version)
URI: http://hdl.handle.net/2115/33763
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 加茂 直樹

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